Fig 1: The mTOR regulates the localization and activity of PepT2 in bovine mammary epithelial cells (BMEC). (A, C) The analysis of PepT2 in plasma membrane or whole lysate of BMEC treated with or without rapamycin (100 ng/mL) for 12 h (A) or DEPTOR siRNA was detected by Western blot (C). Upper panel: representative blot; bottom panel: quantitative representation. (B, D) The uptake of ß-Ala-Lys-AMCA treated with or without rapamycin (B) or DEPTOR siRNA (D) was measured at 25 µmol/L, pH 6.5, and 37 °C for 30 min. Values are the means ± SD (n = 3). Means with a * indicates significant difference (P < 0.05). mTOR = mammalian target of the rapamycin; PepT2 = peptide transporter 2; BMEC = bovine mammary epithelial cells; DEPTOR = DEP domain-containing mTOR-interacting protein; ß-Ala-Lys-AMCA = ß-Ala-Lys-N-7-amino-4-methylcoumarin-3-acetic acid.
Supplier Page from Abcam for Anti-Deptor antibody