Fig 1: miR-2116-3p exerted its function through mediation on SERPINA3 in GBM. A CCK-8 assay was performed to detect cell viability. B EdU assay was used to detect cell proliferation. C The protein levels of Bax, Bcl-2, cleaved-caspase-3, caspase-3 in GBM cells were detected by western blotting. (A-C) U251 and A172 cells were transfected with si-SERPINA3, miR-2116-3p inhibitor, si-SERPINA3 plus miR-2116-3p inhibitor or negative control. *P < 0.05, **P < 0.001 compared with blank group. #P < 0.05, ##P < 0.001 compared with Si-SERPINA3 + inhibitor group. Data were present as mean ± SD
Fig 2: SERPINA3 was a target of miR-2116-3p. A The potential binding sites between miR-2116-3p and SERPINA3. B Dual-luciferase reporter assay was performed to verify the relationship between miR-2116-3p and SERPINA3. **P < 0.001 compared with miR-2116-3p mimic NC. C SERPINA3 could be obviously pulled down by miR-2116-3p mimic showed by RNA pull-down analysis. **P < 0.001 compared with bio-mimic-NC. D Upregulation of SERPINA3 in GBM tissues. **P < 0.001 compared with normal tissues. (E) Negative correlation between miR-2116-3p and SERPINA3 expression. F Upregulation of SERPINA3 in U251 and A172 cells. **P < 0.001 compared with NHA. G The transfection efficiency of si-SERPINA3 in U251 and A172 cells by western blotting. *P < 0.05, **P < 0.001 compared with blank group. #P < 0.05, ##P < 0.001 compared with Si-SERPINA3 + inhibitor group. Data were present as mean ± SD
Fig 3: SERPINA3 facilitated GBM cell migration and invasion. A Wound healing assay was used to detect cell migration. B Cell invasion was detected by transwell assay. (A-B) U251 and A172 cells were transfected with si-SERPINA3, miR-2116-3p inhibitor, si-SERPINA3 plus miR-2116-3p inhibitor or negative control. *P < 0.05, **P < 0.001 compared with blank group. #P < 0.05, ##P < 0.001 compared with Si-SERPINA3 + inhibitor group. Data were present as mean ± SD
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