Fig 1: Immunocytochemical staining of seeded UCs (I) and SMCs (II) on ASC-based self-assembled scaffold. The expression of UPIa, UPII, CK7, and CK20 by UCs (IA,IC,IE,IG) and a-SMA, MHC and smoothelin by SMCs (IIA,IIC,IIE), are detected in red color. No UC and SMC marker expression was detected in negative control. 4’,6-diamidino-2-phenylindole (DAPI) was used as nuclear counterstain in blue color. The blue color detected in negative control (I and II), represents nuclei of UC (IB,ID,IF,IH) and ASCs (IIB,IID,IIF). The scale bar represents 100 µm. Results are from a representative of three independent experiments.
Fig 2: VSP inhibited the levels of UP Ia and UP Ib in the mouse bladder. (A), Representative costained images with UP Ia, UP Ib, and DAPI captured the bladder tissue of the UPEC CFT073-infected C3H/HeN mice. Yellow rectangle: higher magnification view of the selected area (parallel sections, the green signals represent UP Ia, the red signals represent UP Ib, the blue signals represent DAPI, scale bar = 50 µm); (B), Representative scatterplots for the UPIa and UPIb expression levels from the bladder tissue of the C3H/HeN mice infected with UPEC CFT073. (C, D), Statistical analysis of the UPIa- and UPIb-positive cells in the entire cross-sectional tissue region of the mouse bladder. Quantitatively, the percentage of cells expressing UPIa (UPIa/DAPI) and UPIb (UPIb/DAPI). n=3 mice per group, compared with the control group: # p < 0.05. The results were determined by Wilcoxon rank-sum test.
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