Fig 2: CD34 immunohistochemistry in the intestinal phase of (a) Group 1 (Control non-infected group), (b) Group 2 (Infected non-treated group), (c) Group 3 (Albendazole-treated group), (d) Group 4 (Curcumin 150mg-treated group), (e) Group 5 (Curcumin 300mg-treated group), (f) Group 6 (Combined Curcumin 150 mg + Albendazole group) showing marked increase in CD34 immuno-expression in the inflammatory cells and capillaries of the infected group. Albendazole and curcumin treatment showed a reduction in the CD34 immuno-reactive capillaries and inflammatory cells (Scale bar 50μm).

Fig 3: CD34 immunohistochemistry in the muscle phase of (a) Group 1 (Control non-infected group), (b) Group 2 (Infected non-treated group), (c) Group 3 (Albendazole-treated group), (d) Group 4 (Curcumin 150mg/kg-treated group), (e) Group 5 (Curcumin 300mg/Kg-treated group), (f) Group 6 (Combined Curcumin 150 mg/kg + Albendazole group) showing marked increase in CD34 immunohistochemistry in the dense inflammatory cellular infiltration and capillary endothelial surrounding the encysted larvae (L) in the infected group. The albendazole-treated group shows moderate CD34 expression in the epimysium surrounding a degenerated larva (L). The curcumin 150 mg/Kg group and the curcumin 300 mg/Kg group show moderate CD34 immunohistochemical expression in the capillary network and inflammatory cells invading the disintegrated larvae. The combined curcumin 150 mg/kg + albendazole group shows few CD34 positive capillaries around homogenized larvae (L) with degenerated capsules (Scale bar 50μm).

Fig 4: Representative images of Foxp3, CD34 and CD45 immunoreactive cells in the S-LN. In morphine group, significant increases of S-LN Foxp3 (regulatory T cell marker), and decreases of CD34 (general stem cell marker) and CD45 (hematopoietic stem cell marker) immunolabeled cells were demonstrated as compared to those of normal, however, these morphine-induced increases of Foxp3 and decreases of CD34 and CD45 immunoreactivity cells were obviously and significantly normalized by HT7, SI5 and LI5 acupunctures, in that orders. (a) normal; (b) M 10; (c) M 10 + HT7 acupuncture; (d) M 10 + SI5 acupuncture; (e) M 10 + LI5 acupuncture. All ABC based immunohistochemistric eosin stain. Scale bars: 80 µm. S-LN : Submandibular lymph node; Foxp3: Forkhead box P3; CD : Cluster of differentiation; ABC : Avidin-biotin-peroxidase complex.

Fig 5: Immunofluorescence assessment of endothelialisation of grafts functionalized with distinct RGD peptides or unmodified prostheses. (A) Double immunostaining for CD31 (red, mature endothelial cells) and CD34 (green, endothelial progenitor cells) with DAPI (blue, nuclei) counterstaining, representative confocal microscopy images. Scale bar = 20 μm. G means graft, L means lumen. (B) CD31+ cell count. Whiskers indicate range, boxes bounds indicate 25th and 75th percentiles, centre lines indicate median. One-way ANOVA with Tukey’s multiple comparisons test. (C) CD34+ cell count. Whiskers indicate range, boxes bounds indicate 25th and 75th percentiles, centre lines indicate median. One-way ANOVA with Tukey’s multiple comparisons test. (D) Semi-quantitative analysis of endothelial phenotype based on CD31+ and CD34+ cell count.