Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody ab194528 from Abcam

Product Specs
ItemAnti-IKK alpha + IKK beta (phospho S176 + S177) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab194528
Quantity100 UL
ApplicationsWestern blot
Conjugate/TagUnconjugated
IsotypeIgG
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
NCBI Gene AliasesIKK-beta, IKK2, IKKB, IMD15, NFKBIKB
ModificationsPhospho-S177
Gene NameIKBKB
ReactivityMouse
TargetIKK alpha + IKK beta

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Website: http://www.abcam.com

(1) Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injuryCellular & Molecular Biology LettersNovember 9, 2023Xinyu Ling, Shiyou Wei, Dandan Ling, Siqi Cao, Rui Chang, Qiuyun Wang, Zhize Yuan1:1000,"ab76302, Abcam","WB, IHC, IF" Ikbkb,1:1000,"ab178870, Abcam",WB phos-Ikbkb,1:50 ~ 1:1000,"ab194528, Abcam","WB, IHC, IF" GAPDH,1:1000,"MA1-16,757, ThermoFisher",WB Goat anti-rabbit IgG H&L (HRP),"1:20,000","ab205718,

(2) Cinnamaldehyde Induces the Expression of MicroRNA-1285-5p and MicroRNA-140-5p in Chondrocytes to Ameliorate the Apoptosis and Inflammatory ResponseCARTILAGESeptember 1, 2023Gang Li, Yun Songanti-IKKα/β antibody (1:1,000, ab178870; Abcam Inc.), and anti-phospho-IKKα/β antibody (p-IKKα/β; 1:1,000, ab194528; Abcam Inc.) overnight at 4 °C and the membranes were subsequently incubated with horseradish peroxidase–conjugated

(3) CX3CL1 promotes M1 macrophage polarization and osteoclast differentiation through NF-κB signaling pathway in ankylosing spondylitis in vitroJournal of Translational MedicineAugust 25, 2023Xinzhe Feng, Shanbang Zhu, Junjie Qiao, Zhou Ji, Bole Zhou, Weidong Xuincluding iNOS (ab178945, Abcam, USA), ACP5 (EPR21791, Abcam, USA), p-P65 (ab264271, Abcam, USA), p-IKKα/β (ab194528, Abcam, USA), p-IkBα/β (ab133462, Abcam, USA) and GAPDH (ab8245, Abcam, USA), at 4 ℃ overnight. After

(4) Ubiquitin-specific protease 11 Aggravates Ischemia-reperfusion-induced Cardiomyocyte Pyroptosis and Injury by Promoting TRAF3 DeubiquitinationBalkan Medical JournalMay 10, 2023Yunfei Zhang, Juledezi Hailati, Xiaoyun Ma, Hasidaer Midilibieke, Zhiqiang LiuAbcam), anti-IL-1β (1:1000, ab300501, Abcam), anti-IL-18 (1:1000, ab207323, Abcam), anti-p-IKKβ (1:1000, ab194528, Abcam), anti-IKKβ (1:500, ab32135, Abcam), anti-p-NF-kB (1:1000, ab76302, Abcam), anti-NF-kB (1:1000,

(5) Effects of Fish Oil, Lipid Mediators, Derived from Docosahexaenoic Acid, and Their Co-Treatment against Lipid Metabolism Dysfunction and Inflammation in HFD Mice and HepG2 CellsNutrientsJanuary 13, 2023Yan Su, Hack-Sun Choi, Jong-Hyun Choi, Hee-Sik Kim, Gil-Yong Lee, Hee-Won Cho, Heonsik Choi, Yong-Suk Jang, Jeong-Woo Seokit was purchased from Sango Company (San Diego, CA, USA). Antibodies against IKKB (ab32135), p-IKKB (ab194528), IKB (ab32518), p-IKB (ab133462), NF-kB (p65) (ab16502), p-NF-kB (p-p65) (ab76302), and GAPDH (ab181602)

(6) Regulation of Atherosclerosis by Toll-Like Receptor 4 Induced by Serum Amyloid 1: A Systematic In Vitro StudyBioMed Research InternationalSeptember 15, 2022Jinhui Chen, Gang Liu, Yan Hong, Jing Han, Zhe Yang, Yanping Yang, Hong Li, Shumin Wang, Lili Jue, Qi Wangwith the following primary antibodies: MyD88 (1 : 2000, ab219413, Abcam, USA); P-IKKα/β (1 : 1500, ab194528, Abcam, USA); inhibitor of nuclear factor κB kinase subunit α/β (IKKα/β, 1 : 1000, ab178870, Abcam,

(7) Knockdown of triggering receptor expressed on myeloid cells 1 (TREM1) inhibits endoplasmic reticulum stress and reduces extracellular matrix degradation and the apoptosis of human nucleus pulposus cellsExperimental and Therapeutic MedicineJuly 29, 2022Ji Zhang, Haoran Jiang, Min Li, Lixiang DingBiotechnology cleaved-caspase3,ab32042,Rabbit,1:500,Abcam cleaved-PARP,ab32064,Rabbit,"1:5,000",Abcam TLR4,ab13556,Rabbit,1:500,Abcam MyD88,ab133739,Rabbit,"1:5,000",Abcam p-IKK,ab194528,Rabbit,"1:1,000",Abcam IKK,ab97406,Rabbit,"1:1,000",Abcam p-p65,AF5875,Rabbit,"1:1,000",Beyotime Institute

(8) TNF-Î± induces AQP4 overexpression in astrocytes through the NF-ÎºB pathway causing cellular edema and apoptosisBioscience ReportsMarch 31, 2022Hong Lu, Li Ai, Baoyue Zhang5% bovine serum albumin (BSA, ST023, Beyotime, China) for 2 h, antibodies against P-IKKα/β (1:100, ab194528, Abcam, U.S.A.), IKKα (1:500, ab38515, Abcam, U.S.A.), IKKβ (1:500, ab124957, Abcam, U.S.A.), P-IκBα

(9) NEK2 enhances malignancies of glioblastoma via NIK/NF-κB pathwayCell Death & DiseaseJanuary 14, 2022Jianyang Xiang, Wahafu Alafate, Wei Wu, Yichang Wang, Xiaodong Li, Wanfu Xie, Xiaobin Bai, Ruichun Li, Maode Wang, Jia Wangpurchased from Abcam (ab227958). Anti-p-IKKα/β (Ser176/177) primary antibody was purchased from Abcam (ab194528). Anti-p52 primary antibody was purchased from Thermo Fisher Scientific (MA5-17217). Anti-SNAIL primary

(10) Serum‑derived exosomes from house dust mite‑sensitized guinea pigs contribute to inflammation in BEAS‑2B cells via the TLR4‑NF‑κB pathwayMolecular medicine reportsNovember 1, 2021Liu C, Huang XL, Liang JP, Zhong X, Wei ZF, Dai LX, Wang J.antibodies against CD63 (cat. no. ab68418), IKKα/β (cat. no. ab178870), phosphorylated (p)-IKKα/β (cat. no. ab194528) and HSP70 (cat. no. ab31010) were procured from Abcam, anti-TLR4 (cat. no. BA1717) was from Boster

(11) Proteomics identifies a novel role of fibrinogen-like protein 1 in Crohn's diseaseWorld journal of gastroenterologySeptember 21, 2021Sun XL, Qiao LC, Gong J, Wen K, Xu ZZ, Yang BL.ab32041, Abcam, United Kingdom), IKKβ (1:1000 dilution, ab32135, Abcam), p-IKKα/β (1:1000 dilution, ab194528, Abcam), IκBα (1:1000 dilution, ab32518, Abcam), p-IκBα (1:1000 dilution, ab133462, Abcam), NF-κB (p65,

(12) Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in micePharmaceutical BiologyJune 3, 2021Jianbin Sun et al.(ab81283), phospho-p38 (ab4822), phospho-cPLA2 (ab53105), phospho-5-LO (ab30573) and phospho-IKKα/β (ab194528) were obtained from Abcam (Cambridge, UK). A specific antibody against PI3K (sc-1637) was procured from

(13) The Protection of Crocin Against Ulcerative Colitis and Colorectal Cancer via Suppression of NF-κB-Mediated InflammationFrontiers in PharmacologyMarch 18, 2021Shanshan Teng, Jie Hao, Hui Bi, Congcong Li, Yongfeng Zhang, Yaqin Zhang, Weiwei Han, Di Wang(dilution: 1:2000) (catalog number: bs-18129R), P-IKK (α/β) (85/87 kDa) (dilution: 1:1000) (catalog number: ab194528; Abcam, Cambridge, MA, USA), Bcl-2 associated X protein (Bax) (21 kDa) (dilution: 1:2000) (catalog number:

(14) Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-κB signalingOncogeneFebruary 1, 2021Wang Z, Shi Y, Ying C, Jiang Y, Hu J.#ab16502, Abcam), p-IκBα (1:10000, #ab133462, Abcam), IκBα (1:1000, #ab76429, Abcam), p-IKKα/β (1:500, #ab194528, Abcam), IKKα (1:1000, #ab109749, Abcam), IKKβ (1:500, #ab97406, Abcam), Collagen I (1:1000, #ab34710,

(15) Caffeoyl-Prolyl-Histidine Amide Inhibits Fyn and Alleviates Atopic Dermatitis-Like Phenotypes via Suppression of NF-κB ActivationInternational Journal of Molecular SciencesSeptember 28, 2020Hayan Jeong et al.MA, USA), anti-SYK (1:1000, A2123, Abclonal, Woburn, MA, USA), anti-phospho IKK (S176/177) (1:5000, ab194528, Abcam, Cambridge, UK), anti-IKK (1:5000, ab178870, Abcam, Cambridge, UK), anti-phospho IκB (S32) (1:1000,

(16) SMC1 promotes proliferation and inhibits apoptosis through the NF‑κB signaling pathway in colorectal cancerOncology ReportsAugust 8, 2019Jin Li, Jingdong He, Yuan Wang, Yongqian Shu, Jianwei Zhousubunit β (IKKβ; 1:1,000; cat. no. ab124957; Abcam), phosphorylated (p)IKKβ (Ser177; 1:1,000; cat. no. ab194528; Abcam), inhibitor of nuclear factor-κB subunit α (IκBα; 1:1,000; cat. no. ab32518; Abcam), pIκBα (Ser32;

(17) Cholesterol crystals promote endothelial cell and monocyte interactions via H2O2-mediated PP2A inhibition, NFκB activation and ICAM1 and VCAM1 expressionRedox BiologyJune 1, 2019Prahalathan Pichavaram, Arul M. Mani, Nikhlesh K. Singh, Gadiparthi N. Raopurchased from Santa Cruz Biotechnology (Santa Cruz, CA). Neutralizing anti-ICAM1 (ab171123), pIKKα/β (ab194528) and neutralizing anti-VCAM1 (ab47159) antibodies were obtained from Abcam (Cambridge, MA). Cytostatin

(18) Aloin suppresses lipopolysaccharide‑induced inflammation by inhibiting JAK1‑STAT1/3 activation and ROS production in RAW264.7 cellsInternational Journal of Molecular MedicineJuly 31, 2018Yunfei Ma, Tuo Tang, Lili Sheng, Ziqian Wang, Hong Tao, Qing Zhang, Yao Zhang, Zhilin Qipurchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The anti-phospho-IκB (IKK; S176/177, ab194528) antibody was purchased from Abcam (Cambridge, UK). Secondary antibodies coupled to IRDye 800 fluorophore

(19) Suppressive effects of Wang‑Bi Tablet on adjuvant‑induced arthritis in rats via NF‑κB and STAT3 signaling pathwaysInternational Journal of Molecular MedicineJune 8, 2018Yun‑Yun Guan, Yeqing Zhang, Li‑Xin Liu, Hai‑Dong Li, Dan Xue, Wei‑Lian Bao, Guan Ye, Xiaoyan ShenBiosciences, Franklin Lakes, NJ, USA). Antibodies against phosphorylated (p)‑p65 (ab194926), p(IKK) α/β (ab194528), NF‑κ B inhibitor α (Iκ Bα) (ab32518), Toll‑like receptor 4 (TLR4) (ab13867) and p‑signal transducer and

(20) Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signalingOncotargetDecember 29, 2017Deng H, Kuang P, Cui H, Luo Q, Liu H, Lu Y, Fang J, Zuo Z, Deng J, Li Y, et al.overnight at 4°C with the following primary antibodies: mouse NF-κB (ab32536), IκB (ab32518) and IKK (ab194528) antibodies from Abcam, Cambridge, UK; mouse Bc1-2 (3498T), Bclxl (2764T), Bax (14796S), Bak (12105T),

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Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody from Abcam

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Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody from Abcam

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