Fig 1: TRIM58 interacted with RECQL4 and enhanced its ubiquitination in CRC cells. A The relative mRNA levels of RECQL4 in SW620 cells transduced with RECQL4 expression vector. B The protein levels of RECQL4 in SW620 cells transduced with RECQL4 expression vector and treated with MG132. C Co-IP assay shows that TRIM58 interacted with RECQL4 in SW620 cells. D Ubiquitination assay was performed to examine the ubiquitination of RECQL4 in LOVO cells transduced with TRIM58 shRNA and SW620 cells transduced with TRIM58 expression vector. E Immunohistochemistry staining was performed to examine the protein expression of TRIM58 and RECQL4 in CRC and paracancerous tissues (scale bar, 100 μm)
Fig 2: Overexpression of RECQL4 abolished the effect of TRIM58 overexpression in CRC cells. SW620 cells were transduced with TRIM58 and/or RECQL4 expression vector. A Cell viability was measured using CCK-8 assay. B, C Cell cycle was measured through flow cytometry. D Protein levels of AKT, p-AKT, p21, and cyclin D1 were measured through Western blotting. *p < 0.05, ***p < 0.001 vs. oeNC. #p < 0.05, ###p < 0.001 vs. oeTRIM58
Fig 3: TRIM58 inhibited tumor growth in vivo. A, B Overexpression of TRIM58 inhibited tumor growth in vivo. C Western blot was used to examine the relative protein levels of TRIM58 and RECQL4 in tumors. *p < 0.05, ***p < 0.001 vs. oeNC
Supplier Page from Abcam for Anti-RECQL4 antibody