Fig 1: Protein analyses at the muscle endplate region. a Representative images of muscle endplates from YA vs VO (VL muscle) and WT vs Sarco (gastrocnemius muscle) labeled with primary antibodies against phospho-MuSK (scale bars = 10 μm). b Semi-quantitative analysis of protein intensities at the muscle endplate for rapsyn and AChRs in YA (n = 154–158 endplates) vs VO (n = 304–311 endplates) (VL muscle) and WT (n = 185 endplates) vs Sarco (n = 287–296 endplates) (gastrocnemius muscle). Values are normalized as a fold change of YA or WT, respectively. *P < 0.05 vs corresponding group within the same species
Fig 2: Morphological and transcriptional markers of denervation. a Abundance of very small fibers in YA vs VO rat vastus lateralis muscle, WT vs Sarco mouse gastrocnemius muscle, and WT vs Sarco mouse soleus muscle. b qPCR analysis of denervation markers: MuSK, AChRa, AChRe, AChRg, cPLA2, NCAM, and RUNX1 in YA (n = 8) vs VO (n = 10) rat vastus lateralis muscle and WT (n = 8) vs Sarco mouse (n = 6) gastrocnemius and soleus muscle. TBP was used as endogenous control. Data are shown as means with standard error. T tests were performed. *P < 0.05 was considered as statistically significant
Supplier Page from Abcam for Anti-MUSK (phospho Y755) antibody