Fig 1: Assembly state of cV, cII, and cIV in ?4-CYB cells Complexome profiles of the two cV structural and assembly modules. The graphs were generated as in Fig 3, but in this case, the peptide intensity values for the individual subunits belonging to each module (He et al, 2018) were averaged to simplify the analysis. The represented values are the mean ± SEM of the two reciprocal labeling experiments.Complexome profiles of the three detected cII subunits. The graphs were generated as in Fig 3. The represented values are the mean ± SEM of the two reciprocal labeling experiments.Complexome profiles of the different cIV assembly modules and of the last subunit to be incorporated (NDUFA4). The graphs were generated as in Fig 3, but in this case, the peptide intensity values for the individual subunits belonging to each assembly module (Vidoni et al, 2017) were averaged to simplify the analysis. The represented values are the mean ± SEM of the two reciprocal labeling experiments. The bar graph represents the quantification of the total area under the curve (calculated as in Fig 3C) in the profiles corresponding to each cIV module. The plotted values are mean ± SD (n = 2). Two-way ANOVA with Sidak's multiple comparisons test **P = 0.0088 (Early); **P = 0.0063 (MT-CO2); **P = 0.0040 (NDUFA4); *P = 0.0264.1D BNGE, Western blot, and immunodetection of two cIV subunits (MT-CO1 and COX7B) in samples from WT and ?4-CYB cells solubilized with DDM and digitonin (Dig). Source data are available online for this figure.