Fig 1: FAT1 inhibit the invasion of human esophageal cancer cell lines. Magnification: 200 X. The Transwell method was used to detect the invasion ability of cells in each experimental group (het-1A cell line, TE13 cell line, TE13 cells treated with cisplatin, and TE13 cells transfected with ov-FAT1 and si-FAT or treated with cisplatin at the same time). The activation of FAT1 inhibits the invasion of human esophageal cancer cell lines. Magnification: 200 X.
Fig 2: FAT1 inhibits the adhesion of human esophageal cancer cell lines. Fibronectin-coated dishes were used to detect the characteristics of cell adhesion to extracellular matrix in each experimental group (het-1A cell line, TE13 cell line, TE13 cells treated with cisplatin, and TE13 cells transfected with ov-FAT1 and si-FAT or treated with cisplatin at the same time). Magnification: 200 X.
Fig 3: FAT1 inhibits the cell viability of human esophageal cancer cell lines. Clone formation assay was used to detect the proliferation ability of cells in each experimental group (het-1A cell line, TE13 cell line, TE13 cells treated with cisplatin, and TE13 cells transfected with ov-FAT1 and si-FAT or treated with cisplatin at the same time). The clones were fixed and stained using Crystal Violet.
Fig 4: The mRNA expression of Yap1 PRPN14 and FAT1 in esophageal cancer. (a) Yap1 mRNA was highly expressed in the tissues of esophageal carcinoma (n = 20), but low in esophageal carcinoma adjacent tissues (n = 20). The expression of PTPN14 (b) and FAT1 (c) was low in the tissues of esophageal carcinoma, but high in the adjacent tissues of esophageal carcinoma. (d) FAT1 was highly expressed in the SEG1, but low in TE13. ***P < 0.001 vs. the EC tissue. ###P < 0.001 vs. TE13.
Fig 5: Western blot was performed to detect the protein levels of Yap1, PTPN14, FAT1, and Myc. WB was used to evaluate the expression of Yap1, PTPN14, FAT1, and Myc. FAT1 significantly enhanced the protein level of PTPN14 and inhibited the protein levels of Yap1 and Myc. Meanwhile, knockdown of FAT1 significantly downregulated the protein level of PTPN14 and increased the protein levels of Yap1 and Myc. *P < 0.05, ***P < 0.001.
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