Fig 1: Western blot analysis of HCP1, HMOX1, HMOX2, FLVCR, Dcytb, DMT1, ZIP14, ZIP8, FPN1, HEPH and Actin (loading control). Representative immunoblots comparing expression of tested molecules in duodenal samples of healthy individuals at different levels of duodenum: (a) immediately post-bulbar, (b) 1–2 cm below the Vater papilla and (c) at the distal duodenum; are shown
Fig 2: Protein levels of regulators related to iron transport (NRAMP2, Natural resistance associated macrophage protein 2) and iron storage (ferritin) are decreased while expression of proteins participating in non transferrin bound iron (NTBI) uptake (ZIP14, Zinc importer protein 14) and iron export (ferroportin) does not differ in tumor-initiating cells (TICs)Protein levels of NRAMP2 (A), ferritin (B), zinc transporter protein 14 (C) and ferroportin (D) in the MCF-7 cell line (CTRL) and MCF-7 derived spheres (SPH) are shown. The protein expression was quantified by the image J software from 2 to 5 independent samples, standard error is SEM, p-values lower than 0.05 are denoted with a star and were calculated and plotted in GraphPad prism, using the unpaired t-test.
Fig 3: Expression profile of Dcytb, DMT1, ZIP14 and ZIP8 on mRNA and protein levels in duodenal samples of healthy individuals at different levels of duodenum: (a) immediately post-bulbar, (b) 1–2 cm below the Vater papilla and (c) at the distal duodenum. mRNA levels (A, C, E, G) were determined using quantitative real-time PCR, protein levels (B, D, F, H) were determined using Western blot analysis. Values are presented as relative numbers when comparing with the expression in immediately post-bulbar duodenum, that is, the column a (1.0). Mean values ± SEM are depicted for b and c. Statistically significant differences (t-test) are indicated by *(p < 0.05), **(p < 0.01)
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