Fig 1: Impaired mitochondrial import complex proteins in ALS i-motor neurons. (a) Mitochondrial protein was isolated from i-motor neurons and run on SDS/PAGE followed by immunoblotting to investigate import machinery proteins TOMM70, TOMM40, TOMM20, TIMM23, and TIMM17A. Representative blots for each protein, cropped to show the protein band of interest along with its individual loading control (VDAC1) is shown. (b) Densitometry was performed using LiCor Image Studio 2.1 software; n = 3 per line, with 3 unique human lines per group. Each data point is the mean value for an individual line relative to a control line from the same blot, and the bar represents the mean of the three lines +/- SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Statistical analyses were performed with Prism software GraphPad version 7.03.
Fig 2: Neurogenic hypertension induces shifts in cardiac mitochondrial dynamics and protein transport. (A) Measurement of relative d-LOOP/GAPDH DNA ratio by qPCR from control and NG-HTN hearts. (B,C) Western blot representative and quantification showing expression of FIS1 and OPA1 level, receptively, normalized to Actin in control and NG-HTN hearts. (D) Measurement of relative d-LOOP/GAPDH DNA ratio by qPCR in control and NE-treated H9c2. (E,F) Representative Western blot and quantification showing expression of TOM20 and TIM17 level, respectively, normalized to Actin. Values are mean ± SEM, dot represents n = 6 rats, *P < 0.05 vs. Control, Student’s t-test.
Supplier Page from Abcam for Anti-TIMM17A/TIM17 antibody [EPR16098]