Fig 1: Apolipoprotein D (ApoD) stimulated macrophages to promote inflammation. ApoD was higher in plasma of nonsurvivor male mice at myocardial infarction (MI) day 3 (A). D-25 plasma ApoD data (iii) were derived from three different blots run under same conditions. The two other blots are shown in Supplemental Fig. S6. Data were analyzed by unpaired t test. ApoD stimulated expression of proinflammatory genes (Ccl3, Ccl5, Il1ß, Il12a) in peritoneal macrophages isolated from male mice (B). Data were analyzed by paired t test. Image showing experimental design was created with BioRender and published with permission (accessed on 5 January 2022) (26). Arg1, anti-inflammatory arginase 1; Ccl3, C-C motif chemokine ligand 3; Ccl5, C-C motif chemokine ligand 5; Il1ß, interleukin-1ß; Il6, interleukin 6; Il10, interleukin 10; Il12a, interleukin 12a; Mrc1, mannose receptor C-type 1; Tnfa, tumor necrosis factor-a; Tgfß1, transforming growth factor-ß1; Ym1, chitinase-like protein 3. D-25, day -25. n represents number of animals in each group.
Supplier Page from Abcam for Anti-Apo-D antibody