HDAC3 (7G6C5) Mouse mAb 3949S from Cell Signaling Technology

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Frontiers in physiology
January 01 2019

Fig 1: Osteoarthritis rats were treated with RGFP966. Immunohistochemistry (IHC) was performed to measure relative inflammatory proteins in OA. (A,B) Expression of collagen II in CG and OAG + RGFP966 group was higher than OAG by IHC. (A,C) Expression of HDAC3 in CG and OAG + RGFP966 group was lower than OAG by IHC. (A,D) Expression of MMP-13 in CG and OAG + RGFP966 group was lower than OAG by IHC. (A,E) The percentage of NF-kappaB positive cell in CG and OAG + RGFP966 group were lower than OAG by IHC; ∗∗p < 0.01; ∗p < 0.05 vs. CG, ##p < 0.01; #p < 0.05 vs. OAG + RGFP966 group; n = 5 rats for each group in IHC, means with SEM.
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Nutrients
November 27 2020

Fig 2: PEITC regulates HDAC3 in the livers of HFCD-fed mice: Immunoblotting was used to measure the levels of HDAC3. Equal loading of protein was ensured by stripping the immunoblot and reprobing it for histone H3. CON: AIN-93G diet, HFCD: 60% calories from fat + 1% cholesterol, HFCD + G: HFCD + 3 mg/kg/day GA, HFCD + P30: HFCD + 30 mg/kg/day PEITC, HFCD + P75: HFCD + 75 mg/kg/day PEITC.
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Breast Cancer Research
June 01 2014

Fig 3: Interactions between JMJD2A, E2F1, E2F4, histone deacetylase (HDAC)1 and HDAC3. (A) JMJD2A could immunoprecipitate E2F1, E2F4, HDAC1 and HDAC3. (B) JMJD2A could be immunoprecipitated by E2F1 with anti-E2F1 antibody. (C) JMJD2A could be immunoprecipitated by E2F4 with anti-E2F4 antibody. IP, immunoprecipation; Ab, antibody.
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Frontiers in cellular neuroscience
January 01 2022

Fig 4: EA treatment reversed WD-TBI-induced elevations in HDAC1 and HDAC3 expression in murine cortex tissue. (A) Timeline for the study procedures including TBI, anesthesia, EA, EA-NAP, and recovery. Mice were sacrificed 48 h after brain injury. (B) Representative Western blot results show levels of HDAC1, HDAC3, and HDAC2 protein expression in murine cortex 48 h after WD-TBI. The brains were removed and the entire cortex was dissected for Western blotting analysis as described in the “Materials and methods.” β-Actin was used as the loading control. (C) Quantitative analysis of HDAC1/3/2 protein expression levels in (B). (D) Representative images of IHC staining show anti-HDAC3 antibody immunoreactivity in the cortical and hippocampal areas of the Control, WD, WD+EA, and WD+EA-NAP groups. High magnification images from the top panels (red box) are shown in the green and blue boxes, respectively. Scale bars of red box panels are 500 μm; of the green and blue box panels are 100 μm. Data are the means ± S.E.M. of at least three independent experiments. *p < 0.05, **p < 0.01 (statistical testing was performed by one-way ANOVA/Newman-Keuls test). The number underneath each bar in (C) refers to the number of mice used in the study group. The original Western blot images are presented in Supplementary Figure 4. The number underneath each bar in (C) refers to the number of mice used in the study group. The F values of one-way ANOVA for (C) are presented in Supplementary Table 2.
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Nature neuroscience
November 01 2016

Fig 5: FOXO3 and HDAC3 recruitment to gene regulatory regions is abrogated in NPCs harboring a patient-derived MECP2 mutation(a) NPC lines were generated using iPSCs derived from a healthy control (C1) and an RTT patient with a heterozygous MECP2R306C mutation (line 4 and 14). To generate isogenic control NPCs (lines 6 and 36), the R306C mutation in the RTT patient-derived line was changed to the wild-type sequence by CRISPR/Cas9 gene editing. (b) ChIP of HDAC3 and IgG followed by qPCR at the promoters of ARRDC2, KLF10, TLE1, BDNF and NR4A1 in healthy (C1), MECP2R306C (line 4, line 14), and isogenic control NPCs (line 6, line 36) (normalized % input to C1). One-way ANOVA; ARRDC2, F(5,29) = 14.79, **** P < 0.0001; KLF10, F(5,29) = 31.08, **** P < 0.0001; TLE1, F(5,29) = 12.65, **** P < 0.0001; BDNF, F(5,29) = 28.76, **** P < 0.0001; NR4A1, F(5,29) = 9.570, **** P < 0.0001. Bonferroni post hoc; C1 versus line 4: ARRDC2, * P = 0.0132; KLF10, *** P = 0.0002; TLE1, * P = 0.0154; BDNF, **** P < 0.0001; NR4A1, * P = 0.0221; C1 versus line 14: ARRDC2, ** P = 0.0024; KLF10, **** P < 0.0001; TLE1, ** P = 0.0018; BDNF, **** P < 0.0001; NR4A1, * P = 0.0345; C1 versus line 6: ARRDC2, P = 0.2027; KLF10, P > 0.9999; TLE1, P > 0.9999; BDNF, P = 0.8822; NR4A1, P = 0.4572; C1 versus line 36: ARRDC2, P > 0.9999; KLF10, P > 0.9999; TLE1, P > 0.9999; BDNF, P > 0.9999; NR4A1, P > 0.9999; C1 versus IgG: ARRDC2, **** P < 0.0001; KLF10, **** P < 0.0001; TLE1, **** P < 0.0001; BDNF, **** P < 0.0001; NR4A1, *** P = 0.0002; n = 10, 5, 5, 4, 4, 7 cell pellets from 4 cultures. (c) Immunostaining of C1 (control), line 4 and line 14 (MECP2R306C), and line 6 and 36 (isogenic control) NPCs using Hoechst (blue) and anti-acFOXO (red). Imaged using confocal microscope; Scale bar, 20 µm; n = 3 coverslips per line. (d) Quantitation of acFoxo immunostaining in NPCs (c). One-way ANOVA; F(4,795) = 39.15, **** P < 0.0001. Bonferroni post hoc; C1 versus line 4: **** P < 0.0001, C1 versus line 14: **** P < 0.0001, C1 versus line 6: P = 0.4427, C1 versus line 36: * P = 0.0174; n = 160 nuclei per line. (e) ChIP of FOXO3 and IgG followed by qPCR at the promoters of ARRDC2, KLF10, TLE1, BDNF and NR4A1 in healthy (C1), MECP2R306C (line 4, line 14), and isogenic control NPCs (line 6, line 36) (normalized % input to C1). One-way ANOVA; ARRDC2, F(5,26) = 11.38, **** P < 0.0001; KLF10, F(5,26) = 19.39, **** P < 0.0001; TLE1, F(5,26) = 16.96, **** P < 0.0001; BDNF, F(5,26) = 15.59, **** P < 0.0001; NR4A1, F(5,26) = 27.79, **** P < 0.0001. Bonferroni post hoc; C1 versus line 4: ARRDC2, ** P = 0.0052; KLF10, * P = 0.0158; TLE1, P = 0.0746; BDNF, ** P = 0.0020; NR4A1, *** P = 0.0005; C1 versus line 14: ARRDC2, ** P = 0.0071; KLF10, * P = 0.0249; TLE1, P = 0.1697; BDNF, ** P = 0.0042; NR4A1, ** P = 0.0098; C1 versus line 6: ARRDC2, P > 0.9999; KLF10, ** P = 0.0042; TLE1, * P = 0.0279; BDNF, P = 0.3387; NR4A1, ** P = 0.0020; C1 versus line 36: ARRDC2, P > 0.9999; KLF10, P = 0.0961; TLE1, * P = 0.0173; BDNF, P = 0.7238; NR4A1, ** P = 0.0037; C1 versus IgG: ARRDC2, *** P = 0.0004; KLF10, *** P = 0.0004; TLE1, *** P = 0.0005; BDNF, *** P = 0.0002; NR4A1, *** P = 0.0002; n = 7, 5, 5, 4, 4, 7 cell pellets from 4 cultures. (f) qRT-PCR analysis of RNA from healthy control (C1), MECP2R306C (line 4, line 14), and isogenic control (line 6, line 36) NPCs for ARRDC2, KLF10, TLE1, BDNF and NR4A1 (Log2 fold change to C1, normalized to GAPDH). One-way ANOVA; ARRDC2, F(4,25) = 22.57, **** P < 0.0001; KLF10, F(4,25) = 23.57, **** P < 0.0001; TLE1, F(4,25) = 23.62, **** P < 0.0001; BDNF, F(4,25) = 9.01, **** P < 0.0001; NR4A1, F(2,25) = 48.89, **** P < 0.0001. Bonferroni post hoc; C1 versus line 4: ARRDC2, P = 0.0582; KLF10, * P = 0.0192; TLE1, ** P = 0.0026; BDNF, * P = 0.0118; NR4A1, P > 0.9999. C1 versus line 14: ARRDC2, * P = 0.0358; KLF10, ** P = 0.0067; TLE1, P = 0.2614; BDNF, * P = 0.0396; NR4A1, P > 0.9999; C1 versus line 6: ARRDC2, * P = 0.0.165; KLF10, *** P = 0.0009; TLE1, * P = 0.0189; BDNF, P > 0.9999; NR4A1, **** P < 0.0001; C1 versus line 36: ARRDC2, *** P = 0.0004; KLF10, P = 0.0611; TLE1, *** P = 0.0007; BDNF, P = 0.3875; NR4A1, **** P < 0.0001; n = 6, 6, 6, 6, 6 cell pellets from 2 cultures. b, e, f, report mean value ± s.e.m. d, report median, 25th and 75th percentile, min and max value.

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HDAC3 (7G6C5) Mouse mAb

Cell Signaling Technology's HDAC3 (7G6C5) Mouse mAb is a Mouse monoclonal antibody. The HDAC3 (7G6C5) Mouse mAb Antibody was generated using HDAC3, and Histone Deacetylase 3 as the antigen. It reacts with Homo Sapiens, Human, Mouse, Rat, and Non-Human Primate. This antibody has been shown to work in applications such as: Precipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot.

Description

HDAC3 (7G6C5) Mouse mAb detects endogenous levels of total HDAC3 protein. The antibody does not cross-react with other HDAC proteins

Product Specs
ItemHDAC3 (7G6C5) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3949S
Quantity100 µl
ApplicationsW, IP, IF-IC
ReactivityH, M, R, Mk
TargetHDAC3
HostMouse
NCBI Gene AliasesHD3, KDAC3, RPD3, RPD3-2
ClonalityMonoclonal
NCBI Full Gene Namehistone deacetylase 3
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG2a
Clone7G6C5
Molecular Weight49

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(1) Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progressioniScienceNovember 17, 2023Zhilei Zhang, Yonghua Wang, Zhijuan Liang, Zhaoyuan Meng, Xiangyan Zhang, Guofeng Ma, Yuanbin Chen, Mingxin Zhang, Yinjie Su, Zhiqiang Li, et al.anti-CD44,Cell Signaling,Cat#:5640; RRID:AB_AB_10547133 Mouse monoclonal anti-HDAC3,Cell Signaling,Cat #:3949; RRID:AB_2118371 Rabbit polyclonal anti-HDAC3,Proteintech,Cat #:10255; RRID:AB_2279733 Rabbit polyclonal

(2) Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinomaJournal of Experimental & Clinical Cancer ResearchNovember 16, 2023Jialiang Wang, Xiaoyu Zhang, Xinyi Ma, Dongmei Chen, Meina Cai, Lexin Xiao, Jing Li, Zexuan Huang, Yuehua Huang, Yifan LianCell Signaling Technology, for western blotting), anti-HDAC2 (5113, Cell Signaling Technology), anti-HDAC3 (3949, Cell Signaling Technology), anti-HDAC4 (7628, Cell Signaling Technology), anti-HDAC6 (7558, Cell Signaling Technology

(3) A Novel Long Noncoding RNA in Osteocytes Regulates Bone Formation through the Wnt/β-Catenin Signaling PathwayInternational Journal of Molecular SciencesSeptember 4, 2023Makoto Arai, Hiroki Ochi, Satoko Sunamura, Nobuaki Ito, Masaomi Nangaku, Shu Takeda, Shingo Sato(Cell Signaling Technology, #34589), HDAC2 (Cell Signaling Technology, #5113), HDAC3 (Cell Signaling Technology, #3949), Histone H3 (Abcam, Cambridge, UK, ab1791), Non-phospho-β-catenin (Ser33/37/Thr41) (Cell Signaling Technology

(4) Reduction of class I histone deacetylases ameliorates ER-mitochondria cross-talk in Alzheimer's diseaseAging cellAugust 1, 2023Marinho D, Ferreira IL, Lorenzoni R, Cardoso SM, Santana I, Rego AC.Biovision. Primary antibodies anti‐GRP75 (#2816), anti‐H3 (#9715), anti‐HDAC2 (#2540), and anti‐HDAC3 (#3949) were from Cell Signaling; anti‐InsP3R1 (19962‐1‐AP) from Proteintech; anti‐VDAC1 (ab14734) and anti‐APP

(5) "Nuclear translocation of an aminoacyl-tRNA synthetase may mediate a chronic ""integrated stress response"""Cell reportsJune 27, 2023Jones JA, Wei N, Cui H, Shi Y, Fu G, Rauniyar N, Shapiro R, Morodomi Y, Berenst N, Dumitru CD, et al.,Cell Signaling Technology,Catalog #: 5113; RRID:AB_10624871 HDAC3,Cell Signaling Technology,Catalog #: 3949; RRID:AB_2118371 RbAp46 (OTI5A4),Novus Biologicals,Catalog #: NBP2–01308; RRID:AB_2725199 RbAp48 (13D10),Novus Biologicals

(6) HDAC3 is critical in tumor development and therapeutic resistance in Kras -mutant non–small cell lung cancerScience AdvancesMarch 17, 2023Lillian J. Eichner, Stephanie D. Curtis, Sonja N. Brun, Caroline K. McGuire, Irena Gushterova, Joshua T. Baumgart, Elijah Trefts, Debbie S. Ross, Tammy J. Rymoff, Reuben J. Shawraised in rabbit from (Abcam, ab76013) was used to immunoprecipitate, and HDAC3 raised in mouse (CST-3949) was used to detect coimmunoprecipitated HDAC3. Drugs Entinostat (MS-275) was obtained from Selleck Chemicals

(7) Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt-ChromiumJournal of functional biomaterialsFebruary 26, 2023da C Fernandes CJ, da Silva RAF, de Almeida GS, Ferreira MR, de Morais PB, Bezerra F, Zambuzzi WF.Brazil. Antibodies: HDAC1 (10E2) Mouse mAb #5356; HDAC2 (3F3) Mouse mAb #5113; HDAC3 (7G6C5) Mouse mAb #3949; HDAC6 (D2E5) Rabbit mAb #7558; SirT1 (1F3) Mouse mAb #8469; SAPK/JNK antibody (phospho-Thr180/ Tyr182)

(8) Histone deacetylase inhibitors promote breast cancer metastasis by elevating NEDD9 expressionSignal transduction and targeted therapyJanuary 6, 2023Hu Z, Wei F, Su Y, Wang Y, Shen Y, Fang Y, Ding J, Chen Y.(#2108 S), phosphor-FAK-Tyr397 (#8556 S), FAK (#3285 S), HDAC1 (#34589 T), HDAC2 (#5113 T), HDAC3(#3949 S), HDAC4 (#5392 S), HDAC5 (#20458 S), HDAC6 (#7558 S) were purchased from Cell Signaling Technology

(9) Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 MiceOxidative medicine and cellular longevityJanuary 1, 2023Li R, Liu R, Chen L, Wang G, Qin L, Yu Z, Wan Z.fibrillary acidic protein (GFAP) (cat#3670), β-actin (cat#66009-1-Ig), histone deacetylase 3 (HDAC3) (cat#3949), phospho-AMPKα (Thr172) (cat#2535), and synaptophysin (cat#5461) were from Cell Signaling. Anti-p-Tau

(10) Epigenetic master regulators HDAC1 and HDAC5 control pathobiont Enterobacteria colonization in ileal mucosa of Crohnâ€™s disease patientsGut MicrobesDecember 31, 2022MÃ©lissa Chervy, Adeline Sivignon, Flavie Dambrine, Anthony Buisson, Pierre Sauvanet, Catherine Godfraind, Matthieu Allez, Lionel Le Bourhis, The Remind Group, Nicolas Barnich, et al.Active Motif), anti-HDAC1, anti-HDAC2, anti-HDAC3, anti-HDAC4, anti-HDAC6, anti-HDAC7 (1/2000, #5356, #2540, #3949, #15164, #7612, #33418, Cell Signaling Technologies), anti-HDAC5 (1/2000, #20458, Cell Signaling Technologies

(11) Histone deacetylase 3 facilitates TNFα-mediated NF-κB activation through suppressing CTSB induced RIP1 degradation and is required for host defense against bacterial infectionCell & BioscienceDecember 1, 2022Liping Yang, Shengchuan Chen, Jingyan Xia, Ying Zhou, Linan Peng, Huimin Fan, Yu Han, Lihua Duan, Genhong Cheng, Heng Yang, et al.They were then stained with anti-CTSB antibodies (CST, #31718, 1:100), anti-HDAC3 antibodies (CST, #3949, 1:100) or anti-P65 antibodies (CST, #8242, 1:100) overnight. After washed by PBS three times, they

(12) CREPT Disarms the Inhibitory Activity of HDAC1 on Oncogene Expression to Promote TumorigenesisCancers (Basel)September 30, 2022Yajun Cao, Bobin Ning, Ye Tian, Tingwei Lan, Yunxiang Chu, Fangli Ren, Yinyin Wang, Qingyu Meng, Jun Li, Baoqing Jia, et al.9E10), anti-HDAC1 (CST, Danvers, MA, USA, 34589, 5356; Abcam, Cambridge, UK, ab19845), anti-HDAC3 (CST, 3949; Abcam, ab137704), anti-TCF4 (CST, 2569), anti-H3K27ac (Abcam, ab4729), anti-actin (Sigma, St. Louis,

(13) Confined migration induces heterochromatin formation and alters chromatin accessibilityiScienceSeptember 16, 2022Hsia CR, McAllister J, Hasan O, Judd J, Lee S, Agrawal R, Chang CY, Soloway P, Lammerding J.(Millipore) was added to blocking buffer for certain primary antibodies (mouse anti-HDAC3, Cell Signaling, 3949, 1:250; goat anti-HP1α, Abcam, ab77256, 1:300). For staining of cells in 3D collagen matrices, cells

(14) TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expressionCommunications biologySeptember 9, 2022Yu Z, Wang L, Zhao J, Song H, Zhao C, Zhao W, Jia M.(9167,1:1000), anti-p65 (3031 S,1:1000), anti-HDAC1 (5356,1:1000), anti-HDAC2 (5113,1:1000), anti-HDAC3 (3949,1:1000), and anti-HDAC4 (7628,1:1000) antibodies were purchased from Cell Signaling Technology (Danvers,

(15) WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathwayCell death & diseaseMay 20, 2022Feng Y, Ma Z, Pan M, Xu L, Feng J, Zhang Y, Shao C, Guo K, Duan H, Zhang Y, et al.Proteintech), anti-HDAC1 (1:1000, #5356, CST), anti-HDAC2 (1:1000, #5113, CST), anti-HDAC3 (1:1000, #3949, CST), anti-HDAC4 (1:1000, 17449-1-AP, Proteintech), anti-HDAC5 (1:1000, 16166-1-AP, Proteintech), anti-HDAC6

(16) Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1Cell reportsMay 17, 2022Bowness JS, Nesterova TB, Wei G, Rodermund L, Almeida M, Coker H, Carter EJ, Kadaster A, Brockdorff N.anti-RING1B,Gift from H. Koseki,N/A Mouse monoclonal anti-HDAC3,Cell Signaling Technology,"Cat.No. 3949S, clone 7G6C5, RRID:AB_2118371" Rabbit polyclonal anti-H3K27me3 (ChIP),Diagenode,"C15410069, RRID:AB_2814977" Mouse

(17) Histone deacetylation regulates nucleotide excision repair through an interaction with the XPC proteiniScienceApril 15, 2022Masayuki Kusakabe et al.,Cell Signaling Technology,Cat#: 5113; RRID: AB_10624871 Mouse monoclonal anti-HDAC3 (7G6C5),Cell Signaling Technology,Cat#: 3949; RRID: AB_2118371 Rabbit monoclonal abti-HDAC8,Abcam,Cat#: ab187139; RRID: AB_2715505 Mouse monoclonal

(18) Electroacupuncture improves TBI dysfunction by targeting HDAC overexpression and BDNF-associated Akt/GSK-3β signalingFrontiers in cellular neuroscienceJanuary 1, 2022Hung SY, Chung HY, Luo ST, Chu YT, Chen YH, MacDonald IJ, Chien SY, Kotha P, Yang LY, Hwang LL, et al.Cell Signaling Technology, Danvers, MA, United States), HDAC2 (#5113; Cell Signaling Technology), HDAC3 (#3949; Cell Signaling Technology), ionized calcium-binding adapter molecule 1 (Iba1) (ab5076; Abcam, Cambridge,

(19) Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine CrotonylationDrug design, development and therapyJanuary 1, 2022Chen XF, Ji S.pan anti-Kcr (PTM Biolabs, PTM-501), H3 (Abcam, ab1791), HDAC1 (Proteintech, 10197-1-AP), HDAC3 (CST, 3949), CDYL (Proteintech, 17763-1-AP), GAPDH (Abcam, ab8245) were used in the present study. Statistical

(20) Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activationeLifeDecember 2, 2021Wilfahrt D, Philips RL, Lama J, Kizerwetter M, Shapiro MJ, McCue SA, Kennedy MM, Rajcula MJ, Zeng H, Shapiro VS.RRID:AB_2800047,Flow cytometry (1:1500) Antibody,Anti-HDAC3 (mouse monoclonal),Cell Signaling,Cat #3949S RRID:AB_2118371,Flow cytometry (1:1000) Antibody,Anti-TCRβ FITC (Armenian hamster monoclonal),Biolegend,Cat

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Frontiers in physiology

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HDAC3 (7G6C5) Mouse mAb from Cell Signaling Technology

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HDAC3 (7G6C5) Mouse mAb from Cell Signaling Technology

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