Fig 1: Linoleic acid (C18) and estradiol stimulate FATP1/SLC27A1 expression and estradiol stimulates the binding of ER-ß to FATP1/SLC27A1 promoter (A) FATP1/SLC27A1, (C) ESR1 and (D) ESR2 expression levels in MDA-MB-231 cells in a pulse chase experiment referenced to the control condition. (B) Western blotting for FATP1 detection, the numbers are indicative of fold change of each condition (normalized for the respective ß-actin) in relation to control. (C) ESR1 and (D) ESR2 expression levels in MDA-MB-231 cells in a pulse chase experiment referenced to the control condition. In relative qPCR, hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene was used as housekeeping gene. (E) Relative occupancy of ER-a and ER-ß at FATP1/SLC27A1 promoter. Cells were cultured in control and in estradiol conditions and ER-a and ER-ß binding was assessed by ChIP. Data are mean ± error bars of biological triplicates, only adherent cells were analyzed, dead cells in culture media were discarded. (F) Kaplan-Meier survival curves for patients with grade 3, TNBC (n = 77) and grade 3, luminal A BC (n = 75) for the ESR2 gene. *p < 0.05 **p < 0.01 ***p < 0.001. (*) represents the statistical analysis in relation to control condition (dot line). For pulse chase qPCR experiments a Two-way ANOVA with Tukey’s test was used.
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