Fig 1: Glial cell types and development.(A) tSNE plot showing the regional differences of astrocytes in CC and pons. The expression levels of typical markers AQP4 and GFAP are shown in the bottom. (B) Costaining of NEUROD2 and GFAP in the CC and pons at GW20. NEUROD2 positive cells represent for neurons and GFAP-positive cells represent for astrocytes. The pons contains much larger abundance of astrocytes. NEUROD2, neuronal differentiation 2. (C) Expression levels of oligo subtype markers are shown by boxplot. OLIG1 is a pan marker for all oligo subtypes, PDGFRA lacks expression in Oligo_4, CDK1 is specifically expressed by Oligo_1 cells, APOD lacks expression in Oligo_2, and MOG and PDGFA are specifically expressed by Oligo_4 cells. (D) Co-immunostaining of APOD and PDGFRA in the CC at GW17. The white arrowheads show the double positive cells. DAPI, 4',6-diamidino-2-phenylindole; PDGFRA, platelet-derived growth factor receptor A. (E) Pseudotime map of the subtypes of oligodendrocytes and their progenitors. The regional identity for each cell is also shown on the bottom.
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