Fig 1: Inhibition of HSPG2 and NF-kB reverses the effects of TNFSF13 on HSF fibrosis and inflammation. The expression of fibrosis-associated genes α-SMA, FN1, and COL1A1 in HSF after (A) transfection with si-HSPG2 or (C) co-treatment with TNFSF13 and PDTC were detected by qRT-PCR. The expression of fibrosis-associated proteins α-SMA, Fibronectin, and Collagen I in HSF after (B) transfection with si-HSPG2 or (D) co-treatment with TNFSF13 and PDTC were detected by Western blot. The expression of inflammation cytokines IL-1β, TNF-α, and TGF-β1 in HSF after (E) transfection with si-HSPG2 or (G) co-treatment with TNFSF13 and PDTC were detected by qRT-PCR. The expression of inflammation cytokines IL-1β, TNF-α, and TGF-β1 in HSF after (F) transfection with si-HSPG2 or (H) co-treatment with TNFSF13 and PDTC were detected by Western blot. *P<0.05, **P<0.01.
Fig 2: The function of TNFSF13 on the proliferation and migration of HSF. The cell viability of HSF (A) after treatment with different doses of TNFSF13 and (B) transfection with TNFSF13 siRNAs was detected by CCK-8 assay. The proliferation of HSF (C) after treatment with different doses of TNFSF13 and (D) transfection with TNFSF13 siRNAs was detected by EdU staining. The cell migration of HSF (E) after treatment with different doses of TNFSF13 and (F) transfection with TNFSF13 siRNAs was detected by Transwell assay. *P<0.05, **P<0.01.
Supplier Page from Abcam for Anti-APRIL/TNFSF13 antibody