Fig 1: Effect of prebiotics on the expression of galectin-9, filaggrin, and TSLP in the mesenteric lymph nodes (MLN).C: Untreated control, N: AD control (DNCB-induced), P: Zyrtec-positive control (DNCB-induced + Zyrtec), T1: scGOS/lcFOS (DNCB-induced + scGOS/lcFOS), T2: Inulin (DNCB-induced + Inulin), T3: ß-glucan (DNCB-induced + ß- glucan); *p < 0.05 versus control group, **p < 0.01 versus control group, #p < 0.05 versus negative control group, ##p < 0.01 versus negative control group. Data represent means ± SD of 4 replicates.
Fig 2: Prebiotics increase TLR-9 and Gal-9 protein expression in HT29 cell lines (A, B, C).Western blot analysis showed a dose-dependent increase in TLR-9 and Gal-9 protein levels in HT29 cells following treatment with scGOS/lcFOS (9:1 w/v mixture), inulin, and ß-glucan for 12 h, respectively.
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