Fig 1: STC1 regulates the expression levels of STC1, p-SMAD2/3 and SMAD4 in vivo. (A and B) Silencing of STC1 inhibited the growth of tumors in vivo. (C) The expression levels of STC1, p-SMAD2/3 and SMAD4 were detected using immunohistochemistry. Error bars represent the standard error. *P<0.05. STC1, stanniocalcin-1.
Fig 2: Western blot analysis. (A) The correlation between STC1 and TGFß1 was analyzed. (B) The expression levels of STC1, P-SMAD2/3 and SMAD4 were detected via western blotting. *P<0.05 vs. respective LV3-NC group. STC1, stanniocalcin-1; TGFß1, tumor growth factor ß1. TPM, transcripts per million.
Fig 3: miR-34a regulates the expression of SMAD4 and STC1. (A) Silencing STC1 affected the expression levels of various miRNAs in LN229 cells. (B) Overexpression of STC1 regulated the expression level of STC1 in T98G cells. (C) Effect of miR-34a mimics and inhibitors. (D and E) miR-34a regulated the expression of STC1 and SMAD4. (F) A dual luciferase reporter gene assay was performed. Error bars represent the standard error. *P<0.05. miRNA, microRNA; miR, microRNA; STC1, stanniocalcin-1; NC, negative control; 3'UTR, 3' untranslated region.
Fig 4: The expression profile of AAT knockdown related genes(A) Heat-map of genes altered by AAT knockdown. (B) QPCR was performed to measure the mRNA expression of AAT, Id1, Id2, Id3, Id4, Smad2, Smad3 and Smad4 in HPT-8, HUVEC and JEG-3 cells. (C) Western blot was performed to measure the protein expression of AAT, Id1, Id2, Id3, Id4, Smad2, Smad3 and Smad4 in HPT-8, HUVEC and JEG-3 cells. Right panel, Quantification of the bands.
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