Fig 1: Visual defects after pde6c knockdown.a) Effects of pde6c knockdown at 4 dpf. Pde6c protein levels were suppressed by pde6c morpholino oligonucleotides (MO) as shown by Western blotting. b) The 4 dpf control larvae displayed a significant visual response to the 4x repeated rotating red cross. In contrast, the pde6c morpholino-injected larvae did not show a significant response to the visual stimuli. c) Effects of pde6c knockdown at 5 dpf. The control larvae and pde6c larvae display a robust response to the rotating red cross at 5 dpf. Black * = p<0.01, cw vs. ccw in control larvae. Red * = p<0.01, cw vs. ccw in pde6c larvae (two-tailed t-test with unequal variance for pairwise comparisons, n = 35 wells in the control group, n = 33 wells in the pde6c group). R = red cross, cw = clockwise rotation of cross, ccw = counter-clockwise rotation of cross. The average response to clockwise stimuli minus the average response to counter-clockwise stimuli is shown as a separate point in the graph (cw-ccw). These averages are significantly different between the control and pde6c groups at a 95% confidence limit (p<0.05). The 6-well plates contained 1 larva per well.
Supplier Page from Abcam for Anti-PDE6C antibody