Fig 1: Atf1 is expressed in mouse developing teeth. (A,B) Sagittal section of mouse embryo at E12.5. (C) Proliferation (*) of the oral epithelium (OE) into the ectomesenchyma, (EM) consistent with the formation of a molar tooth bud at early bud stage. ATF1 expression was detected the oral epithelium, as well as in the condensed ectomesenchymal cells. (D) Panoramic photomicrography showing the mouse oral cavity (OC) with tooth germs at early bell stage (E14.5). (E) ATF1 expression was noted in the inner enamel/dental epithelium (IE) and dental papilla (DP). (F–H) At E16.5 (Appendix Figure 1) and E18.5, marked ATF1 expression was observed in the inner enamel epithelium (IE) and in the stratum intermedium (SI), with sparse expression noted in the stellate reticulum (SR) and outer dental (ODE) epithelium. (I–J) In incisor teeth at P0, ATF1 expression was particularly evident in the polarized layer of ameloblasts, and in the Tomes’ processes (TP). (K) Atf1 mRNA detected by whole mount in situ hybridization with digoxigenin-labeled antisense RNA followed by alkaline phosphatase-coupled antibody against digoxigenin in C57BL mice at embryonic day 10.5. Strong expression is noted in the mesenchyme of frontonasal prominences, branchial arches and limbs (Obtained from MGI Gene Expression Database. Original source: Gray et al. Mouse Brain Organization Revealed Through Direct Genome-Scale TF Expression Analysis. Science. 2004 24;306(5705): 2255–2257). Secondary antibody goat anti-rabbit-Alexa 555 for detection of ATF1 and DAPI for nuclear staining. OE = Oral ephitelium; EM = ectomesenchymal cells; OC = oral cavity; T = tongue; IE = inner epithelium; DP = dental papilla; SI = stratum intermedium; SR = stellate reticulum; ODE outer dental ephithelium; AM = ameloblasts; TP = Tomes’ processes; BA = branchial arches; Fnp = frontonasal processes; HL = hind limbs; FL = forward limbs.
Supplier Page from Abcam for Anti-ATF1 antibody