Fig 1: The downregulation of TJP2 expression results in dispersed F-actin and disordered microfilaments. (A) No apparent change of microtubules was observed between the negative control group and siRNA-transfected groups in LO2 cells. siNT is the negative control group; siTJP2 (1573) and siTJP2 (1673) are treatment groups transfected with siRNA (1573) and siRNA (1673), respectively. The red box indicates microtubules labeled with green fluorescence. Microtubules in LO2 cells were visualized by confocal microscopy using an anti-ß-tubulin antibody (Abcam, Cat. #ab195883). (B) The downregulation of TJP2 protein expression resulted in dispersed F-actin and disordered microfilaments in LO2 cells. F-actin (Cytoskeleton Inc., Cat. # PHDG1-A) was labeled with red fluorescence; the nucleus was labeled with blue fluorescence by hochest33342 staining. Merge represents the merged fluorescent image of F-actin and nucleus labeled by red and blue fluorescence. (C) No apparent change of microtubules was observed between the negative control group and siRNA-transfected groups in HepG2 cells. (D) The downregulation of TJP2 protein expression resulted in dispersed F-actin and disordered microfilaments in HepG2 cells. Scale bars, 20 µm.