Fig 1: The effects of MXRA8 on M2 macrophage infiltration in vitro by mediating ferroptosis. (A) The correlation coefficient between MXRA8 and classical phenotype markers of macrophages in the GEO datasets. (B) The expression level of MXRA8 and a M2 macrophage phenotype marker (CSF1R) in GBM and normal brain specimens determined by IHC analysis. (C) Infiltration of M2 macrophages in transfected siNC or siMXRA8 cells (U251 and T98G) after pretreatment with RSL3 (5 µM) and subsequent Fer-1 (20 µM). ***p < 0.001.
Fig 2: MXRA8 was identified as a novel prognostic-related gene in glioma. (A) Flowchart presenting the process of identifying the gene and prognostic nomogram of glioma in this study. (B) Venn diagram to identify differentially expressed genes between glioma and normal brain tissue from the GEO database. The 404 overlapping genes were all upregulated in tumor tissue. The 416 overlapping genes were all downregulated in tumor tissue. (C) Prognostic genes were identified using the least absolute shrinkage and selection operator Cox regression model (LASSO). The generated coefficient distribution plots for the logarithmic (lambda) sequence for the selection of the best parameter (lambda). (D) Heatmap of the correlations between 35 upregulated prognostic genes and the 14 ferroptosis-related DEGs. (E, F) The expression of MXRA8 was compared between normal and GBM tissue in the GSE59612 (E) and GSE147352 (F) cohorts. (G) AUC of time-dependent ROC curves verified the prognostic performance of the risk score based on MARA8 in the TCGA cohort. Three-year survival (H), 5-year survival (I), and 10-year survival (J) curves for the OS of patients in the high-risk and low-risk groups in the TCGA cohort.
Fig 3: The role of MXRA8 in immune microenvironment of glioma. (A) Immune infiltration cells associated with MXRA8 using ssGESA algorithm from the TCGA database. (B–D) Validations of NK cells, macrophages, and neutrophils by the TISIDB database. (E–O) Associations of the MXRA8 expression with chemokines (E, F) and multiple immunoinhibitory molecules (G–O) from the TISIDB database. (P–R) The association between MXRA8 with immune score calculated by ESTIMATE algorithm, based on GSE59612, GSE147352, and TCGA glioma datasets. (S) The Kaplan–Meier curve of immune score on overall survival of TCGA glioma datasets.
Fig 4: Immunohistochemistry at (A) low and (B) high magnification for MXRA8 in lung metastases from MDA-231EV primary tumors. Scales bars are 600 µm for (A) and 100 µm for (B).
Fig 5: The expression of (A) MXRA8 mRNA levels as determined by qRT-PCR and (B,C) MXRA8 protein levels as determined by Western blotting. MXRA8 mRNA levels were normalized to HPRT, while MXRA8 protein levels were normalized using GAPDH. (C) Quantitation of the MXRA8 band intensities from the Western blot relative to the GAPDH band intensities. In both A and C, the individual values have been indicated along with the mean ± SEM. A Student’s t-test was performed and significance is indicated as * p < 0.05; *** p < 0.001.
Supplier Page from Abcam for Anti-MXRA8/Asp3 antibody