Fig 1: TargetScan, mirDIP, and miRDB databases were used to predict the target genes of miR-1307. (a) miRDB and TargetScan databases were used to select the intersection of predicted target genes of miR-1307. (b) mirDIP database showed the comprehensive binding scores between AGAP1 gene and miR-1307.
Fig 2: miR-1307 inhibited the expression of AGAP1 gene and the expression levels of AGAP1 in OS cell lines and human OS tissues. (a, b) The mRNA levels of AGAP1 after OS cells transfected with PBS, miR-NC mimics, or miR-1307 mimics were assayed by qRT-PCR. (c) The protein levels of AGAP1 after U2OS cells transfected with PBS, miR-NC mimics, or miR-1307 mimics were assayed by western blot. (d) Relative protein level of AGAP1. (e) The mRNA levels of AGAP1 in two OS cell lines and hFOB1.19 cell line were assayed by qRT-PCR. (f) The mRNA levels of AGAP1 in 18 paired human OS tissues and adjacent normal tissues were assayed by qRT-PCR. (g) The protein level of AGAP1 in hFOB1.19 cells and U2OS OS cells was assayed by western blot. (h) Relative protein level of AGAP1. ∗∗P < 0.01.
Fig 3: miR-1307 directly targeted the 3'-UTR of AGAP1 in OS cells. (a) The complementary sequence between the 3'-UTR of AGAP1 and miR-1307. (b) The wild 3'-UTR of AGAP1 (WT) and mutant 3'-UTR of AGAP1 (MUT) were designed and mutated sites were labeled with red areas. (c, d) Luciferase technology is used to assay the luciferase activity after OS cells transfected with miR-NC mimics, miR-1307 mimics, or miR-1307 inhibitor. ∗P < 0.05.
Fig 4: miR-1307 regulated the growth of OS cells via targeting AGAP1. (a, b) Effects on proliferation of OS cells after OS cells transfected with miR-NC mimics, miR-1307 mimics, or miR-1307 mimics+AGAP1 protein. (c–h) Effects on migration and invasion of OS cells after OS cells transfected with miR-NC mimics, miR-1307 mimics, or miR-1307 mimics+AGAP1 protein. Scale bars, 100 μm. ∗P < 0.05, ∗∗P < 0.01.
Supplier Page from Abcam for Anti-AGAP1 antibody