Fig 1: MiR-136 suppressed cell proliferation, migration and invasion, and improved cell sensitivity to PTX and induced cell apoptosis by binding to CBX2 in ovarian cancer. (A and B) The impacts between miR-136 and CBX2 on the IC50 value of PTX were determined by MTT assay in PTX-resistant SKOV3 and HeyA8 cells. (C-E) The effects between miR-136 and CBX2 overexpression on the proliferation of PTX-resistant SKOV3 and HeyA8 cells were determined by CCK-8 and colony formation assays. (F) Flow cytometry analysis was performed to investigate the influences between miR-136 and CBX2 on the apoptosis of PTX-resistant SKOV3 and HeyA8 cells. (G and H) Transwell assay was carried out to investigate the effects between miR-136 and CBX2 on the migration and invasion of PTX-resistant SKOV3 and HeyA8 cells. *P < 0.05
Fig 2: MiR-136 bound to CBX2 in PTX-resistant SKOV3 and HeyA8 cells. (A) Starbase online database was employed to predict the binding sites between miR-136 and CBX2. (B and C) Luciferase activity was detected by dual-luciferase reporter assay in PTX-resistant SKOV3 and HeyA8 cells. (D-G) The mRNA and protein expressions of CBX2 were detected by qRT-PCR and western blot in PTX-resistant ovarian cancer tissues (N = 20), PTX-sensitive ovarian cancer tissues (N = 19), SKOV3 and HeyA8 cells as well as PTX-resistant SKOV3 and HeyA8 cells. (H and I) The effects of circ_0061140 knockdown on the mRNA and protein expressions of CBX2 were demonstrated by qRT-PCR and western blot, respectively, in PTX-resistant SKOV3 and HeyA8 cells. (J) Spearman correlation analysis was conducted to elucidate the linear relationship between CBX2 and circ_0061140 expressions. (K-M) The influences between miR-136 mimic and CBX2 on the mRNA and protein expressions of CBX2 were demonstrated by qRT-PCR and western blot, respectively, in PTX-resistant SKOV3 and HeyA8 cells. *P < 0.05
Fig 3: Circ_0061140 silencing enhanced the inhibitory effects of PTX on tumor growth in vivo. (A and B) The effects of circ_0061140 absence on the volume and weight of tumors after PTX treatment were revealed. (C and D) QRT-PCR was employed to determine the impacts of circ_0061140 silencing on the expressions of circ_0061140 and miR-136 under PTX exposure in vivo. (E and F) The mRNA and protein expressions of CBX2 were uncovered by qRT-PCR and western blot, respectively, in forming tumors from sh-NC, sh-NC + PTX, sh-circ_0061140 or sh-circ_0061140 + PTX group. *P < 0.05
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