Fig 1: Cell-to-cell interaction and gene expression enrichment analyses. (A) Comparison of the overall information flow of each signaling pathway between tumor-free and tumor-invaded TDLNs with B cells as signal receiver, and significant signaling pathways were ranked based on differences in the overall information flow. (B) Dot plots showing comparison of the communication probabilities mediated by ligand-receptor pairs between tumor-free and tumor-invaded TDLNs with B cells as signal receiver. (C) Bar plots showing altered hallmark pathways scored by GSVA in B lymphocytes from tumor-invaded TDLNs. (D) Bar plots showing enrichment of genes differentially expressed by B lymphocytes from tumor-invaded TDLNs through Gene Ontology analysis of biological process. (E) Violin plots showing the gene expression distribution of IFN-γ among major cell types from TDLNs and between GC− patients and GC+ patients. (F) Western blot analysis of TDLNs from footpad xenograft of NSCLC between control group and NK cell-depleted group. (G) Representative images of IF staining for NCR1 and IFN-γ in human TDLNs. Ctrl, control; GC, germinal center; GSVA, gene set variation analysis; IF, immunofluorescence; IFN, interferon; NK, natural killer; NSCLC, non-small cell lung cancer; TDLN, tumor-draining lymph node.