Fig 1: Associations between the DEGs and patient outcomes in liver cancer. Overall survival curve for patients with liver cancer in the GEPIA database (right) and overall survival and disease specific survival curves (left, center) for patients with liver cancer in the Kaplan-Meier plotter databases stratified by expression of (A) POSTN, (B) LAYN, (C) HTRA3, (D) AANAT, (E) AFM, and (F) NAPB.
Fig 2: TMAO and TNF-a alter expression of the DEGs in Hepa1-6 cells and Huh7 cells. (A–D) Expression of the POSTN, NAPB, LAYN and HTRA3 mRNAs were significantly upregulated in Hepa1-6 cells and Huh7 cells treated with 50 ng/ml TNF-a and 50 µM TMAO in combination compared to cells treated with 50 ng/ml TNF-a alone; results are mean ± SD (n = 3 per group); Student’s t-test. (E,F) On the contrary, 50 ng/ml TNF-a and 50 µM TMAO in combination significantly downregulated the expression of AANAT and AFM compared to cells treated with 50 ng/ml TNF-a alone; results are mean ± SD (n = 3 per group); Student’s t-test.
Fig 3: Correlations between the DEGs and immune cell infiltration in liver hepatocellular carcinoma (LIHC). (A–C) The expression levels of POSTN, HTRA3 and AANAT significantly positively correlated with the levels of neutrophil and Treg infiltration and negatively correlated with the levels of activated NK cell and CD8+ T cell infiltration. (D,E) The expression levels of NAPB and LAYN significantly positively correlated with neutrophil, Treg, activated NK cell and CD8+ T cell infiltration. (F) The expression levels of AFM negatively correlated with the levels of neutrophil, Treg and activated NK cell infiltration.
Supplier Page from Abcam for Anti-LAYN antibody [EPR11875(2)]