Fig 1: Expression of marker proteins of CAF by flow cytometry: Expression of the six positives (CD155, CD90, SMA-A, S100A4, FAP-A) and two negatives (EpCAM and CD31) markers of CAF in the established TCAFs and NCAFs from the T and TAN samples from two patients; one bearing non-aggressive CAFs (A,B) and bearing aggressive CAFs (C,D) by flow cytometry. (D) compares the flow cytometry expression of SMA and S100A with the WB expression (inset) of SMA and S100A from the same representative aggressive CAFs (TCAF and NCAF). The expressions of PD-L1, and TGFB, by WB, are also presented. The protein expression of the same markers (SMA and S100A4) by WB (inset) from a representative aggressive early passage NCAF and TCAF. The expressions of other CAF markers, such as PD-L1 and TGFB, by WB are also presented with the corresponding densitometric analyses (bar diagrams) using Image J (E). Beta-actin was used as the loading control. The isotype controls for the respective protein are marked as open black lines compared to the red area under curves for the proteins. A three-color scale-based conditional formatting of the pattern of % expression of CAF markers of EpCAM, SMA-A, S100A4, and FAP (by flow cytometry) in the pairs of cultured TCAFs and NCAFs (early passages) from 17 representative patients (as presented with color codes) is presented as a heatmap (F). Red bars represent TCAFs and green bars represent NCAFs (E).