Fig 1: Surface GluA2 expression is significantly reduced in Reelin cKO mice after NMDA receptor block. A–C, Slices were treated with AP-5 (50 µm) and picrotoxin (10 µm). Surface to total (S/T) AMPAR subunits (GluA1, GluA2, GluA4) are quantified between Control (Ctrl; n = 6, a = 6) and Reelin cKO (n = 6, a = 6) mice at 6 months of age. Top, Representative Western blots of surface and total GluA subunits, GAPDH was used as loading controls. A, For S/T GluA1 no significant difference was observed between Reelin cKO and Control. (p = 0.52, n = 6, unpaired t test). B, The S/T GluA2 level is decreased in Reelin cKO when compared with Control (p = 0.007, n = 6, unpaired t test). C, No significant changes in surface GluA4 were observed in Reelin cKO mice (p = 0.23, n = 6, unpaired t test). Error bars represent the SEM. (**p < 0.01).