PE/Cyanine7 anti-mouse IFN-gamma 505825 from BioLegend

Product Specs
ItemPE/Cyanine7 anti-mouse IFN-gamma
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number505825
NCBI Gene AliasesIFN-g, If2f, Ifg
CloneXMG1.2
IsotypeIgG1, Kappa
HostRat
Conjugate/TagPE_CYANINE7
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
Quantity25 µg
ReactivityMouse
Antibody TypePrimary
NCBI Full Gene Nameinterferon gamma
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenE. coli-expressed, recombinant mouse IFN-γ
ClonalityMonoclonal
TargetIFN-gamma

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A Pvs25 mRNA vaccine induces complete and durable transmission-blocking immunity to Plasmodium vivaxNPJ VaccinesDecember 14, 2023Nawapol Kunkeaw, Wang Nguitragool, Eizo Takashima, Niwat Kangwanrangsan, Hiromi Muramatsu, Mayumi Tachibana, Tomoko Ishino, Paulo J. C. Lin, Ying K. Tam, Sathit Pichyangkul, et al.Perm/wash buffer (BD Biosciences 51-2091KZ) and incubated with antibodies against IFN-γ (BioLegend 505826) and IL-2 (BioLegend 503808). An unstimulated sample was included as a negative control. Samples stimulated

(2) Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenesSTAR ProtocolsDecember 1, 2023Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing ChenAB_2650923 "PE/Cy7 anti-mouse IFN-γ Antibody, clone XMG1.2, used as 1:20 dilution",BioLegend,Cat# 505826; RRID: AB_2295770 "PE anti-human/mouse Granzyme B Recombinant Antibody, clone QA18A28, used as 1:20

(3) mRNA‐based Vaccines Targeting the T‐cell Epitope‐rich Domain of Epstein Barr Virus Latent Proteins Elicit Robust Anti‐Tumor Immunity in MiceAdvanced ScienceOctober 27, 2023Ge‐Xin Zhao, Guo‐Long Bu, Gang‐Feng Liu, Xiang‐Wei Kong, Cong Sun, Zi‐Qian Li, Dan‐Ling Dai, Hai‐Xia Sun, Yin‐Feng Kang, Guo‐Kai Feng, et al.PE anti‐mouse IL‐2 antibody (BioLegend, Cat# 503803), PE/Cyanine7 anti‐mouse IFN‐γ antibody (BioLegend, Cat# 505825), and BV786 anti‐mouse IL‐4 antibody (BD Biosciences, cat. #564006) at a concentration of 1:100 in Perm/wash buffer

(4) Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunityNature CommunicationsSeptember 15, 2023Yazhong Cui, Yang Miao, Longzhi Cao, Lifang Guo, Yue Cui, Chuanzhe Yan, Zhi Zeng, Mo Xu, Ting HanBV650 (Biolegend, 506333, clone: MP6-XT22, dilution: 1:400), anti-mouse IFNγ-PE/Cyanine7 (Biolegend, 505826, clone: XMG1.2, dilution: 1:400), anti-mouse CD16/CD32 (BioXcell, BE0307, clone: 2.4G2, dilution: 1:400),

(5) IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4+T cellsiScienceAugust 18, 2023Yanyu Zhang, Song Gao, Shifei Yao, Danlin Weng, Yan Wang, Qi Huang, Xuemei Zhang, Hong Wang, Wenchun XuAntibody,Biolegend,Cat# 116027; RRID: AB_2800581 PE-Cyanine 7 anti-mouse IFN-γ Antibody,Biolegend,Cat# 505826; RRID: AB_2295770 Brilliant Violet 421™ anti-mouse IL-5 Antibody,Biolegend,Cat# 504311; RRID: AB_2563161 Brilliant

(6) A binary module for microbiota-mediated regulation of γδ17 cells, hallmarked by microbiota-driven expression of programmed cell death protein 1Cell ReportsAugust 8, 2023Hsin-I Huang, Yue Xue, Mark L. Jewell, Chin Yee Tan, Barbara Theriot, Nupur Aggarwal, Jacob Dockterman, Yang-Ding Lin, Erin A. Schroeder, Donghai Wang, et al.(UC3-10A6),Biolegend,Cat#137707; RRID:AB_10899574 PE/Cyanine7 anti-mouse IFNγ (XMG1.2),Biolegend,Cat#505826; RRID:AB_2295770 PE anti-mouse IL-17A (TC11-18H10.1),Biolegend,Cat#506904; RRID:AB_315464 TruStain

(7) BAT6026, a novel anti-OX40 antibody with enhanced antibody dependent cellular cytotoxicity effect for cancer immunotherapyFrontiers in OncologyJuly 28, 2023Shizhong Liang, Dandan Zheng, Xiong Liu, Xiong Mei, Congcong Zhou, Cuizhen Xiao, Chao Qin, Haitao Yue, Jian Lin, Cuihua Liu, et al.100732), APC/Cyanine7 anti-mouse CD45 (BioLegend, 103116), PE/Cyanine7 anti-mouse IFN-γ (BioLegend, 505826), eFluor™ 450 anti-FoxP3 (Invitrogen, 48-5773-82) and eFluor™ 506 anti-CD4 (Invitrogen, 69-0042-82).

(8) Loss of PBAF promotes expansion and effector differentiation of CD8+ T cells during chronic viral infection and cancerCell ReportsJune 16, 2023Arjun Kharel, Jian Shen, Ryan Brown, Yao Chen, Christine Nguyen, Donia Alson, Theresa Bluemn, Jie Fan, Kexin Gai, Bin Zhang, et al.FITC Donkey anti-rabbit IgG (min. x-reactivity),Biolegend,"Cat# 406403, RRID:AB_893531" PE/Cyanine7 anti-mouse IFN-gamma,Biolegend,"Cat# 505826, RRID:AB_2295770" APC anti-mouse/rat TNF-alpha,Biolegend,"Cat# 506108, RRID:AB_2721315" APC/Cyanine7 anti-mouse CD107a (LAMP-1

(9) Analyzing immune responses to varied mRNA and protein vaccine sequencesNPJ vaccinesJune 5, 2023Park HJ, Bang YJ, Kwon SP, Kwak W, Park SI, Roh G, Bae SH, Kim JY, Kwak HW, Kim Y, et al.USA), CD8-BV605-labeled (Cat: 100744, clone 53-6.7, BioLegend, CA, USA), IFN-γ-PE-Cy7-labeled (Cat: 505825, clone XMG1.2, BioLegend), TNF-α-FITC labeled (Cat: 506303, clone MP6-XT22, BioLegend), IL-2-APC-labeled

(10) Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustionMolecular cellApril 20, 2023Battistello E, Hixon KA, Comstock DE, Collings CK, Chen X, Rodriguez Hernaez J, Lee S, Cervantes KS, Hinkley MM, Ntatsoulis K, et al.506515), APC anti-mouse TNF-α (BioLegend, Cat# 506307), PE/Cyanine7 anti-mouse IFN-γ (BioLegend, Cat# 505825). Annexin staining was performed with APC Annexin V (BioLegend, Cat# 640920). Samples were analyzed

(11) BATF2 promotes HSC myeloid differentiation by amplifying IFN response mediators during chronic infectioniScienceFebruary 17, 2023Le DT, Florez MA, Kus P, Tran BT, Kain B, Zhu Y, Christensen K, Jain A, Malovannaya A, King KY.A2F10)",ebioscience,"Cat#12-1351-82, RRID:AB_465859" "Anti-Mouse IFNγ (PE-Cy7 conjugated, clone XMG1.2)",Biolegend,"Cat#505826, RRID:AB_2295770" "Anti-Mouse TNFα (Brilliant Violet 605 conjugated, clone MP6-XT22)",Biolegend,"Cat#506329,

(12) Loss of Lkb1 in CD11c(+) myeloid cells protects mice from diet-induced obesity while enhancing glucose intolerance and IL-17/IFN-γ imbalanceCellular and molecular life sciences : CMLSFebruary 13, 2023Sun Y, Wang B, Hu Q, Zhang H, Lai X, Wang T, Zhao C, Wang J, Zhang X, Niu Q, et al.PE-Foxp3 (12-5773-82, eBioscience) and antibodies against cytokines, including BV421-CD8, PE-cy7-IFN-γ (505825, Biolegend), Percp-cy5.5-IL-17A (506919, Biolegend), and APC-IL-4 (504105, Biolegend). Cells from the

(13) BRCA1 deficiency in mature CD8(+) T lymphocytes impairs antitumor immunityJournal for immunotherapy of cancerFebruary 1, 2023Wu B, Qi L, Chiang HC, Pan H, Zhang X, Greenbaum A, Stark E, Wang LJ, Chen Y, Haddad BR, et al.permeabilized using a BD Cytofix/Cytoperm kit (BD Biosciences, 554714) and stained with anti-IFNγ (BioLegend, 505826), anti-TNFα (BioLegend, 506314), anti-IL2 (Invitrogen, 45-7021-82), anti-Granzyme B (Invitrogen, 12-8898-82),

(14) Oxygen microcapsules improve immune checkpoint blockade by ameliorating hypoxia condition in pancreatic ductal adenocarcinomaBioactive materialsFebruary 1, 2023Wu J, Wang X, Chen L, Wang J, Zhang J, Tang J, Ji Y, Song J, Wang L, Zhao Y, et al.BioLegend), Fxop3-APC (Clone:FJK-16s; 17-5773-82; 1:600; Thermofisher), IFN-γ-PE-cy7 (Clone:XMG1.2; 505826; 1:600; BioLegend), Granzyme B-BV421 (Clone:QA18A28; 396414; 1:600; BioLegend), F4/80-PE (Clone:T45-2342;

(15) Immunostaining of tumor-infiltrating immune-cell-related markers and cytokines in the tumor fractional radiation model of host PD-L1-deficient miceSTAR ProtocolsDecember 1, 2022Xinrui Zhao, Chunlin Shao(1:200),Biolegend,"Cat # 104427, RRID: AB_830798" PE/Cyanine7 anti-mouse IFN-γ (1:100),Biolegend,"Cat # 505825, RRID: AB_1595591" Purified anti-mouse IFNAR-1,Biolegend,"Cat # 127322, RRID: AB_11149116" TruStain

(16) Interleukin‐23 receptor defines T helper 1‐like regulatory T cells in oral squamous cell carcinomaImmunity, Inflammation and DiseaseDecember 1, 2022Wei Li, Ning An, Mingwei Wang, Xiguo Liu, Zhidan MeiPE/Cyanine7 anti‐mouse IL‐10,505025,JES5‐16E3,BioLegend PE anti‐mouse TGF‐β1,141403,TW7‐16B4,BioLegend PE/Cyanine7 anti‐mouse IFN‐γ,505825,XMG1.2,BioLegend PE anti‐mouse IL‐17A,506903,TC11‐18H10.1,BioLegend PE/Cyanine7 anti‐mouse IL‐4,504117,11B11,BioLegend PE

(17) Ischemic Stroke Impacts the Gut Microbiome, Ileal Epithelial and Immune HomeostasisiScienceNovember 18, 2022Ge Y, Zadeh M, Yang C, Candelario-Jalil E, Mohamadzadeh M.PE anti-mouse IL-17A,BioLegend,Cat# 506904; RRID:AB_315464 PE/Cyanine7 anti-mouse IFN-γ,BioLegend,Cat# 505826; RRID:AB_2295770 FITC anti-mouse IL-10,BioLegend,Cat# 505006; RRID:AB_315360 PE anti-mouse CD326 (EpCAM),Thermo Fisher Scientific

(18) T cell-intrinsic protein kinase D3 is dispensable for the cells’ activationFrontiers in ImmunologyNovember 17, 2022Jiří Koutník, Michael Leitges, Kerstin Siegmund104;109806), CD62L APC-Cy7 (clone MEL-14; 104428), GL7 FITC (clone GL7; 144604), IFN-γ PE-Cy7 (clone XMG1.2; 505826) (from Biolegend), CD4 V500 (clone RM4-5; 560782), CXCR5 biotin (clone 2G8; 551960), Fas A647 (clone

(19) A RORγt(+) cell instructs gut microbiota-specific T(reg) cell differentiationNatureOctober 1, 2022Kedmi R, Najar TA, Mesa KR, Grayson A, Kroehling L, Hao Y, Hao S, Pokrovskii M, Xu M, Talbot J, et al.BD 562467, 1:70;IL-17A AF700 (TC11-18H10.1) BioLegend 506914, 1:200;IFN-γ PE-Cy7 (XMG1.2), BioLegend 505826, 1:200;CD11c PE-Cy7 (N418), BioLegend 117318, 1:400;CD11c BV711 (HL3), BD 563048, 1:100;CD11c TotalSeq™-A0106

(20) Kinetic proofreading through the multi-step activation of the ZAP70 kinase underlies early T cell ligand discriminationNature immunologySeptember 1, 2022Voisinne G, Locard-Paulet M, Froment C, Maturin E, Menoita MG, Girard L, Mellado V, Burlet-Schiltz O, Malissen B, Gonzalez de Peredo A, et al.(Biolegend, catalog no. B209683, clone 145-2C11, DF 1:200) and the anti-IFN-γ (Biolegend, catalog no. 505826, clone XMG1.2, DF 1:600). T cell isolation and short-term expansion OT-I CD8+ T cells were purified

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11/3/2015Great Antibody for Intracellular Staining Phil LangeThis antibody was recommended by a collaborating lab for the detection of IFNg-producing CD8+ T cells after restimulation with non-specific agents or viral peptide.

PE/Cyanine7 anti-mouse IFN-gamma from BioLegend

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PE/Cyanine7 anti-mouse IFN-gamma from BioLegend

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