Fig 2: Progression-free survival (PFS) and the combined FAK/MST1R/c-MET protein profile. (A) PFS was examined for all eight different combinations of FAK/MST1R/c-MET expression levels, with each marker dichotomized based on its median H-score. Kaplan-Meier curves fall into three distinct clusters 1–3. (B) PFS analysis, based on the combined protein profile clusters 1–3, examining all patients. (C) PFS analysis, based on the combined protein profile clusters 1–3, examining stage II patients only.

Fig 3: GFP-LIMK1 fusions increase phosphorylation status of FAK signaling components. (A) Western blot analysis of whole cell lysates from MDA-MB-231 stable transductants probed with antibodies against pFAK, total FAK, pPaxillin, total Paxillin, pSrc, total Src, pAKT, total AKT, pErk1/2 and total Erk 1/2. (B) Western blot analysis for tubulin or GAPDH for each extract used in panel A, as loading controls.

Fig 4: Cell culture in 3D collagen type I gels. (a) Macroscopic view of collagen 3D constructs containing PANC-1 cells; (b) Western blot bands of pFAKY397 and total FAK in PANC-1, MiaPaCa-2 and MCF-7 cells cultured in 0.15% (soft) and 0.5% (stiff) collagen gels; (c) densitometry of bands shown in (b) represented as pFAKY397/FAK; (d) Western blot bands of pFAKY397 and total FAK in PANC-1 cells cultured in 0.15% (soft) and 0.5% (stiff) collagen gels in the presence of GM6001.

Fig 5: Gain and loss of Ang2 in neural crest cultures affects the amount of phosphorylated FAK. Neural tubes electroporated with either a GFP control vector (a), Ang2-FL (b), or Ang2-shRNA (c) were plated on fibronectin/poly-l-lysine coated glass and allowed to grow for 24 h. Electroporated vector in blue. The cultures were then stained with HNK1 (red) and pFAK (green) antibodies. Scale bars 50 µm. Inset images in a, b, and c: individual filopodia in the pFAK channel showing the size measurement. Scale bar 3 µm. d The average pFAK cluster size. Each cluster of pFAK was individually measured by the spot detection algorithm (Imaris) based on the fluorescence in the pFAK channel, so thousands of clusters were used to calculate the average, SEM, and comparison between conditions. Control 4 neural tubes and n = 4179 pFAK clusters, Ang2-FL 4 neural tubes and n = 3182 pFAK clusters, 4 neural tubes and Ang2-shRNA n = 3298 pFAK clusters. All conditions are statistically different from each other with control vs. Ang2-FL p = 1 × 10–22, control vs. Ang2-shRNA p = 5 × 10–85, and Ang2-FL vs. Ang2-shRNA p = 1 × 10–132