Fig 1: Western analysis of Novel Activation Marker, DAB2, in Mouse Microglia. Western blot shows DAB2 levels in A. BV2 mouse microglia and C, primary mouse microglia after treatment with control, LPS, IL-4/IL-13, aBeta-immune complex (aB-IC), or IL-10 (top row). Second and third rows show iNOS and ARG1, respectively, to verify M1, M2a, and M2b simulation. GAPDH was used to normalize the loading across samples (bottom row). Table shows the normalized relative abundance of DAB2 in B. BV2 mouse microglia and D, primary mouse microglia after various treatments as compared with the control sample. GAPDH levels were used to normalize protein abundance in each sample.