Fig 1: Systemic administration of CR8 inhibits isoflurane-induced neuronal cell cycle activation and neuronal apoptosis. (A) Representative immunoblotting of cell cycle and apoptotic proteins in animals exposed to 1.2% isoflurane with or without CR8. (B-G) Quantitative analysis of western blots shows the effect of CR8 on isoflurane anesthesia induced cyclin B1(B), phospho-Cdk1(Thr-161) (C), phospho-Cdk1(Tyr-15)(D), cleaved caspase-3(E), phospho-Bcl-x(F), and cytochrome c (G) levels in the mouse brain, normalized to GAPDH levels. Data presented are mean ± SEM (n = 4-6, *P < 0.05, **P < 0.01, ***P < 0.001 compared with sham; #P < 0.05, ##P < 0.01, ###P < 0.001 compared with Isoflurane)