Fig 1: Biologics co-production with individually controllable biologic expression cassettes. E, estrogen induction; M, methanol induction; E+M, estrogen plus methanol induction. Red text above the gels indicates commercial standards while black text indicates samples obtained under induction with E and/or M. a Schematic illustrating the inducible production of one or two biologics from the dual-biologics production strain. b Titers of hGH and IFNa-2b in the supernatants of P. pastoris under different induction conditions. Values represent mean and s.e.m. (n = 3). c One microgram pure hGH or IFNa-2b or 30 µL supernatant of each sample was loaded in each lane and western blotting was performed with anti-hGH and anti-IFNa-2b antibodies. d The ratio of hGH to IFNa-2b in supernatants increased as the concentration of estrogen increased. e Schematic representation of the strain expressing HSA and hGH. The expression of hGH and the ratio of hGH to HSA in supernatants increased as the concentration of estrogen increased. f Schematic representation of the strain expressing hGH and G-CSF. The expression of G-CSF and the ratio of G-CSF to hGH in supernatants increased as the concentration of estrogen increased. The protein titers and the ratios for protein co-expression were calculated using the Image Lab software based on Coomassie blue staining