Fig 1: Increased expression of genes involved in necrosis in miR-208a KO mice after acute CCl4 treatment. WT or miR-208a KO mice received oil (n = 4-5/group) or CCl4 (n = 6/group) treatment for 48 hours. Whole-liver cell lysates were used to determine the protein levels of Bax (A), CypD (B), PCNA (C), and cyclin D1 (D) by western blot analysis. ß-actin was used as a loading control. Density units are shown in the bar diagram from mice, Bax (n = 4 [oil], n = 6 [CCl4]), CypD (n = 4 [oil]; n = 6 [CCl4]), PCNA (n = 3 [oil], n = 3 [CCl4]), and cyclin D1 (n = 3 [oil], n = 3 [CCl4]). *P < 0.05 versus oil-treated WT mice, # P < 0.05 versus CCl4-treated WT mice.