HA Tag Antibody Alexa Fluor 488 A-21287 from Thermo Fisher Scientific

Product Specs
ItemHA Tag Antibody Alexa Fluor 488
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA-21287
Quantity100 µL
ApplicationsImmunocytochemistry, Western Blot
Conjugate/TagAlexa Fluor™ 488
FormatPBS with 0.1% BSA and 5mM sodium azide; pH 7.2
ReactivityTag
TargetHA Tag
IsotypeIgG1
Clone16B12
HostMouse
Immunogentwelve amino acid peptide CYPYDVPDYASL
ClonalityMonoclonal
Concentration1 mg/mL
Storage Temperature4° C, store in dark
Antibody TypePrimary
Storage4° C, store in dark

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Image-based and machine learning-guided multiplexed serology test for SARS-CoV-2Cell Reports MethodsAugust 22, 2023Vilja Pietiäinen, Minttu Polso, Ede Migh, Christian Guckelsberger, Maria Harmati, Akos Diosdi, Laura Turunen, Antti Hassinen, Swapnil Potdar, Annika Koponen, et al.647",BioLegend,"CAT#652513, RRID:AB_2716153" "Mouse monoclonal anti-HA-tag antibody 16B12, Alexa Fluor 488",Invitrogen,"CAT#A-21287, RRID:AB_2535829" "Goat anti-rabbit IgG(H + L) cross-adsorbed secondary antibody, Alexa Fluor 488",Invitrogen,"CAT#A-11008,

(2) Evolutionarily conserved amino acids in MHC-II mediate bat influenza A virus entry into human cellsPLOS BiologyJuly 6, 2023Okikiola M. Olajide, Maria Kaukab Osman, Jonathan Robert, Susanne Kessler, Lina Kathrin Toews, Thiprampai Thamamongood, Jacques Neefjes, Antoni G. Wrobel, Martin Schwemmle, Kevin Ciminski, et al.565014, 1:200) and Alexa Fluor 488–conjugated HA tag monoclonal antibody (Thermo Fisher, catalog no. A-21287, 1:200) for 30 min on ice. After washing and centrifugation, cells were fixed in 2% PFA in PBS for 20

(3) Autoregulation of GPCR signalling through the third intracellular loopNatureMarch 1, 2023Sadler F, Ma N, Ritt M, Sharma Y, Vaidehi N, Sivaramakrishnan S.fibronectin (F1141) and normal goat serum (G9023) were purchased from Millipore Sigma. anti-HA-Alexa488 (A-21287), formaldehyde (cat# PI28908), and ProLong Diamond Anti-fade Mountant (P36970) were purchased from ThermoFisher.

(4) Influenza A virus reassortment in mammals gives rise to genetically distinct within-host subpopulationsNat CommunNovember 11, 2022Ketaki Ganti, Anish Bagga, Silvia Carnaccini, Lucas M. Ferreri, Ginger Geiger, C. Joaquin Caceres, Brittany Seibert, Yonghai Li, Liping Wang, Taeyong Kwon, et al.viruses were detected in the tissues using a mouse anti-HA Alexa Fluor 488 (Invitrogen catalog number A-21287; clone 16B12; 1:50 dilution) and mouse anti His Alexa Fluor 555 (Invitrogen catalog number MA1-135-A555;

(5) Hermansky-Pudlak syndrome type 1 causes impaired anti-microbial immunity and inflammation due to dysregulated immunometabolismMucosal ImmunologyOctober 27, 2022Athena Cavounidis, Sumeet Pandey, Melania Capitani, Matthias Friedrich, Amy Cross, Lisa Gartner, Dominik Aschenbrenner, Seunghee Kim-Schulze, Ying Ka Lam, Georgina Berridge, et al.1:2000) for 1 h at 37 °C. Cells were stained with an anti-HA AF488 antibody (ThermoFisher, clone 16B12, A21287) for 1 h following fixation and permeabilization with 0.01% Triton for 10 min at room temperature. Cells

(6) Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodieseLifeJanuary 25, 2022Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, et al.AB_2810930,IF (1:500) Antibody,Mouse monoclonal anti-HA-Alexa Fluor 488,Thermo Fisher Scientific,Cat# A-21287 RRID: AB_2535829,IF (1:1000) Antibody,Goat anti-human IgG Fc-HRP,Thermo Fisher Scientific,Cat# A18829

(7) Protocol for crystal structure determination of the antagonist-bound human cannabinoid receptor CB2STAR ProtocolsJune 21, 2021Ling Shen, Xiaoting Li, Junlin Liu, Kaiwen Liu, Zhi-Jie Liu, Tian HuaRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, "HA Epitope Tag Antibody, Alexa Fluor® 488 conjugate (16B12)",Thermo Fisher Scientific Inc.,Cat# A-21287; RRID: AB_2535829 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, DH5a

(8) Genetic Code Expansion and Click-Chemistry Labeling to Visualize GABA-A Receptors by Super-Resolution MicroscopyFrontiers in synaptic neuroscienceJanuary 1, 2021Kuhlemann A, Beliu G, Janzen D, Petrini EM, Taban D, Helmerich DA, Doose S, Bruno M, Barberis A, Villmann C, et al.were incubated with an anti-HA Alexa Fluor 488, anti-HA Alexa Fluor 555, and anti-HA Alexa Fluor 647 (A21287, 26183-A555, and 26183-A647, Thermo Scientific, concentration: 2 μg/ml), respectively, for 30 min on

(9) Development, Screening, and Validation of Camelid‐Derived Nanobodies for Neuroscience ResearchCurrent Protocols in NeuroscienceDecember 1, 2020Clara E. Gavira‐O'Neill, Jie‐Xian Dong, James S. Trimmermilk powder + 0.1 % Triton X-100 Alexa Fluor 488 anti-HA mouse IgG1 mAb 16B12 (ThermoFisher Catalog# A21287) Alexa Fluor 555 anti-mouse IgG subclass-specific antibodies, dependent on the controls used Hoechst

(10) Function-based high-throughput screening for antibody antagonists and agonists against G protein-coupled receptorsCommunications biologyMarch 26, 2020Ren H, Li J, Zhang N, Hu LA, Ma Y, Tagari P, Xu J, Zhang MY.directly staining the cells with Alexa Fluor 488 conjugated anti-HA antibodies (5 μg per ml, Invitrogen, A-21287) at 4 °C for 30 min. The ratio of the GeoMean with sdAb staining relative to the GeoMean with anti-HA

(11) Biased Signaling of the G-Protein-Coupled Receptor β2AR Is Governed by Conformational Exchange KineticsStructureMarch 1, 2020Rajan Lamichhane, Jeffrey J. Liu, Kate L. White, Vsevolod Katritch, Raymond C. Stevens, Kurt Wüthrich, David P. MillarRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies "HA Epitope Tag Antibody, Alexa Fluor 488 conjugate (16B12)",Thermo Fisher Scientific,Cat#A-21287 RRID: AB_2535829 ,, ,, ,, Bacterial and Virus Strains,Bacterial and Virus Strains,Bacterial and

(12) A Discrete Presynaptic Vesicle Cycle for Neuromodulator ReceptorsNeuronFebruary 19, 2020Jullié D, Stoeber M, Sibarita JB, Zieger HL, Bartol TM, Arttamangkul S, Sejnowski TJ, Hosy E, von Zastrow M.anti-FLAG (M1),Sigma,Cat# F-3040; RRID: AB_439712 Anti-HA Alexa-488,Thermo Fisher Scientific,"Cat# A-21287, RRID: AB_2535829" Anti-HA Alexa-594,Thermo Fisher Scientific,"Cat# A-21288, RRID: AB_2535830" "Donkey

(13) A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neuronseLifeSeptember 30, 2019Dong JX, Lee Y, Kirmiz M, Palacio S, Dumitras C, Moreno CM, Sando R, Santana LF, Südhof TC, Gong B, et al.-T, cells were incubated with anti-HA mouse IgG1 mAb 16B12 conjugated to Alexa Fluor 488 (ThermoFisher Cat# A-21287) diluted to 0.67 μg/mL in blocking solution to detect bound nAb, and mouse IgG subclass-specific Alexa

(14) A Comprehensive Drosophila melanogaster Transcription Factor InteractomeCell ReportsApril 16, 2019Leila Shokri et al.used at 20 ng/ml); mouse Alexa488-conjugated anti-HA monoclonal antibody (Thermo Fisher Scientific, A-21287; used at 1 μg/mL); mouse anti-FLAG M2-horse radish peroxidase (HRP)-conjugated antibody (Sigma, A8592;

(15) Crystal Structure of the Human Cannabinoid Receptor CB2CellJanuary 10, 2019Xiaoting Li et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies "HA Epitope Tag Antibody, Alexa Fluor® 488 conjugate (16B12)",Thermo Fisher Scientific Inc.,Cat#A-21287; RRID: AB_2535829 "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant

(16) Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomesNature communicationsNovember 6, 2018Imoto Y, Abe Y, Honsho M, Okumoto K, Ohnuma M, Kuroiwa H, Kuroiwa T, Fujiki Y.The Alexa 488-conjugated mouse anti-HA antibody was purchased from Thermo Fisher Scientific (Cat. #A-21287). A mouse anti-GFP antibody (B2) was purchased from Santa Cruz Biotechnology (Cat. #sc-9996). For immunoblotting,

(17) Monocyte activation detected prior to a diagnosis of schizophrenia in the US Military New Onset Psychosis Project (MNOPP)Schizophrenia researchJuly 1, 2018Weber NS, Gressitt KL, Cowan DN, Niebuhr DW, Yolken RH, Severance EG.Johns Hopkins University School of Medicine; 600 North Wolfe Street; Blalock 1105; Baltimore, MD, U.S.A. 21287 cAgency for Healthcare Research and Quality, Center for Evidence and Practice Improvement, 5600 Fisher

(18) Host Nitric Oxide Disrupts Microbial Cell-to-Cell Communication to Inhibit Staphylococcal VirulenceCell host & microbeMay 9, 2018Urbano R, Karlinsey JE, Libby SJ, Doulias PT, Ischiropoulos H, Warheit-Niemi HI, Liggitt DH, Horswill AR, Fang FC.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies "Anti-HA mouse IgG1, clone 16B12, Alexa Fluor 488 Conjugate",Molecular Probes,Cat#A21287; RRID: AB_2535829 "Anti-HA mouse antibody, monoclonal HA-7",Sigma-Aldrich,Cat#H3663; RRID: AB_262051 7B8

(19) Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin SwitchCellApril 5, 2018Liu N, Hargreaves VV, Zhu Q, Kurland JV, Hong J, Kim W, Sher F, Macias-Trevino C, Rogers JM, Kurita R, et al.acetate on a fresh slide. Protein binding was detected using an anti-HA antibody (Life Technologies, A-21287) or an anti-GST antibody (Life Technologies; catalog, A-11131) at a final concentration of 25 μg/mL

(20) Duplicated TLR5 of zebrafish functions as a heterodimeric receptorProceedings of the National Academy of Sciences of the United States of AmericaApril 3, 2018Voogdt CGP, Wagenaar JA, van Putten JPM.IgG (A11036; Thermo Fisher Scientific); washed with TBS; and incubated (1 h) with Alexa Fluor-488 mouse-α-HA (A21287; Thermo Fisher Scientific). After antibody staining, cells were washed with TBS and MilliQ and embedded

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HA Tag Antibody Alexa Fluor 488 from Thermo Fisher Scientific

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HA Tag Antibody Alexa Fluor 488 from Thermo Fisher Scientific

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