BrdU Antibody Alexa Fluor 647 B35140 from Thermo Fisher Scientific

Product Specs
ItemBrdU Antibody Alexa Fluor 647
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberB35140
Quantity100 Tests
ApplicationsFlow Cytometry
Conjugate/TagAlexa Fluor™ 647
FormatPBS with 5mM sodium azide; pH 7.2
ReactivityChemical
TargetBrdU
IsotypeIgG
CloneMoBU-1
HostMouse
ImmunogenBrdU
ClonalityMonoclonal
Storage Temperature4° C
PurityAffinity chromatography
Antibody TypePrimary
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degronseLifeJune 23, 2022Lewis Macdonald, Gillian C Taylor, Jennifer Margaret Brisbane, Ersi Christodoulou, Lucy Scott, Alex von Kriegsheim, Janet Rossant, Bin Gu, Andrew J Wood/Wash solution, before being incubated with AlexaFluor-647-conjugated anti-BrdU monoclonal antibody (Invitrogen, B35140, 1 in 20 dilution in Perm/Wash, 20-min incubation at room temperature). Perm/Wash (2 ml per sample)

(2) Limited access to antigen drives generation of early B cell memory while restraining the plasmablast responseImmunitySeptember 14, 2021Glaros V, Rauschmeier R, Artemov AV, Reinhardt A, Ols S, Emmanouilidi A, Gustafsson C, You Y, Mirabello C, Björklund ÅK, et al.TY/11.8),BioLegend,Cat#127223; RRID: AB_2716103 AF647 Mouse BrdU (Clone MoBU-1),Thermo Fisher Scientific,Cat#B35140; RRID: AB_2536440 PE Armenian Hamster Anti-Mouse CD11c (Clone N418),BioLegend,Cat#117307; RRID: AB_313776 PE Rat Anti-Mouse F4/80

(3) Effects of trypanocidal drugs on DNA synthesis: new insights into melarsoprol growth inhibitionParasitologySeptember 1, 2021Stephen Larson, McKenzie Carter, Galadriel Hovel-Minerand incubated with anti-BrdU antibody conjugated with Alexa Fluor 647 (1:250 dilution, Thermo Fisher B35140), 1:1000 DAPI (1 mg mL−1) and 0.5% BSA overnight. Prepared cells were then washed with PBS and resuspended

(4) CD73⁺ extracellular vesicles inhibit angiogenesis through adenosine A_2B receptor signallingJournal of extracellular vesiclesJanuary 1, 2020Angioni R, Liboni C, Herkenne S, Sánchez-Rodríguez R, Borile G, Marcuzzi E, Calì B, Muraca M, Viola A.(Sigma Cat#D5025-150KU) for in 1 h at 37°C before the staining with AlexaFluor647 MoBU1-antiBrdU (Cat#B35140 Invitrogen) for 20 min at room temperature. Labelled cells were detected at FACS Canto II. Data analysis

(5) Autoreactive, Low-Affinity T Cells Preferentially Drive Differentiation of Short-Lived Memory B Cells at the Expense of Germinal Center MaintenanceCell reportsDecember 18, 2018Jain RW, Parham KA, Tesfagiorgis Y, Craig HC, Romanchik E, Kerfoot SM.

(6) Roles of human POLD1 and POLD3 in genome stabilityScientific reportsDecember 15, 2016Tumini E, Barroso S, -Calero CP, Aguilera A.for 30 minutes in the same buffer containing 1:25 anti-BrdU antibody conjugated with Alexa Fluor 647 (B35140, Invitrogen) and 0.5 μg/μl RNase A, washed once and resuspended in PBS containing 7AAD (51-68981E, BD)

(7) Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosisThe American journal of pathologyNovember 1, 2015Tsukui T, Ueha S, Shichino S, Inagaki Y, Matsushima K.

(8) Antagonism of Ang-Tie2 and Dll4-Notch signaling has opposing effects on tumor endothelial cell proliferation, evidenced by a new flow cytometry methodLaboratory investigation; a journal of technical methods and pathologyNovember 1, 2014Payton M, Jun T, Wayne W, Yu D, Manoukian R, Chung G, Zhang N, Sun JR, Kaplan-Lefko P, Scully S, et al.studies using a U-87-GFP tumor xenograft model. Mouse anti-BrdUrd-alexa-647 antibodies (cat A21305, cat B35140) were obtained from Life Technologies. Cell Surface, BrdUrd, and DNA Staining Approximately 1 106

(9) Dual-pulse labeling using 5-ethynyl-2'-deoxyuridine (EdU) and 5-bromo-2'-deoxyuridine (BrdU) in flow cytometryCurrent protocols in cytometryJanuary 1, 2011Bradford JA, Clarke ST.

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BrdU Antibody Alexa Fluor 647 from Thermo Fisher Scientific

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BrdU Antibody Alexa Fluor 647 from Thermo Fisher Scientific

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