Fig 1: CXCR5-PD-1+ICOS+ T Cells in HIV-Infected LNs Express TFH-Cell-Related Proteins and Function(A) Histograms showing fluorescent intensity of BCL6, Maf, and CD84 staining in naive (1), CXCR5-PD-1-ICOS- (2), CXCR5-PD-1+ICOS+ (3), and TFH cells (4). Plots summarize data from 8 HIV LNs.(B) Plasma cell phenotype of B cells cocultured with the indicated T cell subsets without or with SEB.(C-E) Quantification of total IgG (n = 13) (C), plasma cells (n = 15) (D), or CD4+ T cells (n = 15) (E) on the 7th day of each coculture condition.(F) IFN-? level in the supernatant of wells containing TFH cells or CXCR5-PD-1+ICOS+ T cells (n = 9).(G) Example gate for T-bet+ B cells. Naive IgD+CD27- B cells were excluded. Scatterplot quantifies T-bet+ B cells in 7 HC and 25 HIV-infected LNs.(H) Correlation between IFN-? expression in CXCR5-PD-1+ICOS+ T cells and T-bet+ B cell frequency in HIV-infected LN samples.For (A) and (C)–(E), Friedman test was performed and corrected using Dunn’s multiple comparisons test. For (F), Wilcoxon matched-pairs signed rank test was used. For (G), Mann-Whitney test was used. For (H), association was measured by Spearman correlation. Data are represented as mean ± SEM.Also see Figures S4D and S5A.