Western blot analysis was performed on whole cell extracts (30 µg lysate) of T47D (Lane 1), A549 (Lane 2), U2OS (Lane 3), K562 (Lane 4), HeLa (Lane 5), Raji (lane 6), HEK-MSR (lane 7), MCF7 (lane 8), MDA-MB-231 (lane 9), LNCaP (lane 10) and Hep G2 (lane 11). The blots were probed with Anti-PRMT3 Mouse Monoclonal Antibody (Product # 730020, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 59 kDa band corresponding to PRMT3 was observed across cell lines tested expect Raji and HEK-MSR. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).