Fig 1: Expression of cell-type-specific markers in cells isolated from skin samples(A) Immunofluorescence detection of cell type specific markers. Cells were seeded on coverslips, fixed, permeabilized and stained with appropriate antibodies. Each coverslip was incubated with two different antibodies raised in different hosts and then the signal was pseudocolored green. The scale bar represents 100 µm. The following antibodies were used for immunofluorescence: Keratin 14 (KRT14), Abcam (ab7800, dilution 1:100); Tyrosinase Related Protein 1 (TYRP1), Covance (SIG-38150, dilution 1:500); Vimentin (VIM), Santa Cruz (sc-6260, dilution 1:100); Platelet Derived Growth Factor Receptor Alpha (PDGFRa), Abcam (ab203491, dilution 1:500); Cadherin 5 (CDH5), Santa Cruz (sc-9989, dilution 1:100); Platelet And Endothelial Cell Adhesion Molecule 1 (PECAM1), Millipore (04-1074, dilution 1:100); CD34 Molecule (CD34), Abcam (ab81289, dilution 1:100).(B) FACS analysis of CD34 expression (CD34 antibody, BD Biosciences, cat. no. 550760, dilution 1:50). Cells were trypsinized and fixed without permeabilization to detect membrane bound CD34 protein. Blue color represents control with only secondary antibody, pink shows CD34 expression.