Fig 2: Several domains of OmpP2 are sufficient for CAV1, ARF6, and PPP2R1A interactions. (A) OmpP2 protein structural representation for the standard reference strain G. parasuis serotype 5. (B) Schematic of the OmpP2 domain architecture and constructs used in domain mapping studies. (C) Immunoblot using anti-Flag MAb for Flag-CAV1, Flag-ARF6, and Flag-PPP2R1A recombinant proteins from transfected HEK 293T cells and mutant proteins.

Fig 3: Knockdown of CAV1, ARF6, and PPP2R1A impairs the adhesion and phagocytosis of G. parasuis by iPAM cells. (A) siRNA silencing and Western blot assessment of CAV1, ARF6, and PPP2R1A protein levels. (B) Ability of G. parasuis to adhere to iPAM-silenced cells. Error bars represent SD of three independent experiments performed in triplicate. **, P < 0.01; ***, P < 0.001. (C) Phagocytosis ability of G. parasuis by iPAM-silenced cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001.

Fig 4: Overexpression of CAV1, ARF6, and PPP2R1A promoted the adhesion and phagocytosis of G. parasuis by iPAM cells. (A) Transfection of the expression plasmids and Western blot assessment of CAV1, ARF6, and PPP2R1A protein levels. (B) Ability of G. parasuis to adhere to transfected iPAM cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001. (C) Phagocytosis of G. parasuis by transfected iPAM cells. Error bars represent SD of three independent experiments performed in triplicate. ***, P < 0.001.

Fig 5: A model for venlafaxine (VEN) antagonizing the NE-promoted colon cancer progression by inhibiting NET.By binding with beta-adrenergic receptor (b-AR), norepinephrine (NE) induces Akt activation, VEGF expression, cell proliferation, angiogenesis, and cancer progression in human colorectal cancer (CRC) cells. NE also increases the expression of NE transporter (NET). Venlafaxine (VEN) can antagonize the above effects of NE by inhibiting the NE-increased NET expression, which is related to the interaction of NET and protein phosphatase 2 scaffold subunit alpha (PPP2R1A).