Fig 1: Protrusions of invading cells contain increased levels of GTP and guanylate biosynthesis enzymes.a Schematic representation of the chamber used for separation of cell bodies (CBs) and cell protrusions (CPs) (left), and representative images and orthoslices of CB and CP acquired in one of the two GFP channels (right). b Representative false-colored radiometric images of cell bodies and cell protrusions in MDA-MB-231 and SK-Mel-103 cells transduced with GEVAL30 or GEVALNull (GTP Evaluator). c Quantification of GEVAL activity in MDA-MB-231 and SK-Mel-103 cells transduced with GEVAL30 (G30) or GEVALNul (GNull). Data is average ± SEM of 3 independent experiments (30 CB and 30 CP analyzed per experiment). Statistics was performed by a two-tailed paired Student’s t-test. d Cell bodies (CB) and cell protrusions (CP) were collected as described in Methods followed by protein quantification and probing of equal amounts of material in immunoblotting with the designated antibodies (left) or staining with Coomassie blue (right). Please note that GMPR is not expressed in SK-Mel-103 cells27 and was excluded from the analysis. An approximate ratio of CB and CP materials is shown. Shown are representative gel images of at least two independent experiments.