Fig 1: Levels and distribution of DISC1 and NF-?B in the hippocampus of He–/– mice. (A) Immunoblotting for DISC1 and Tubulin as a loading control in the striatum (Str, n = 9/genotype) or hippocampus (Hipp, n = 10–11/genotype) of 8-week-old He+/+ and He–/– mice. Densitometry quantification in the striatal (B) and hippocampal (C) samples of results as in (A). Data were normalized to tubulin for each sample and expressed as percentage of wild type. (D) Representative confocal images of NF-?B immunofluorescence in CA1 (40x objective) of 8-week-old He+/+ and He–/– mice (n = 10 He+/+ and 8 He–/–). Scale bar = 70 microns. (E) Quantification of NF-?B the IOD per layer (total IOD mean per layer). Bars represent mean ± SEM. Data were analyzed by Student’s t-test. ***p < 0.001 when compared with He+/+ mice in (E).