Fig 2: TDRD3 is recruited to virus-induced stress granules.(A) HeLa cells were infected (MOI = 1) for 6–16 hr with indicated enteroviruses before fixation and processing for IFA with antibodies to G3BP1 and TDRD3. (B) Cells infected with PV were stained for IRF7 and G3BP1 or cells infected with EVD-68 were stained for TRAF3 and G3BP1.

Fig 3: IFN-I effectors are recruited to G3BP1-induced and various stress-induced SGs.(A-C) U20S cells were treated to produce oxidative shock, heat shock or osmotic shock SGs as described in the Materials and Methods, then fixed and stained for IRF3, IRF7, TBK1, TRAF3, Sting or cGAS. IFA was then performed using deconvolution microscopy. (D) G3BP1-GFP was transiently expressed in U20S cells for 18 hrs to allow formation of SGs, which were then subjected to IFA microscopy for innate factors indicated as described in the Materials and Methods.