Fig 2: Representative IHC staining of CRYAB expression in colorectal cancer tissues and normal colorectal tissues.Representative histopathological sections of (A–C) normal colorectal tissues and (D–F) colorectal cancer tissues stained with IHC. The colon section was incubated with HRP-labeled goat anti-pika universal antibody and stained with DAB (brown). Images are shown at 200 × magnification (scale bar, 100 µm). IHC: immunohistochemistry. (G) We cut three sets of immunohistochemical sections, including three normal tissues and three cancer tissues, and tested the number of positive cells.

Fig 3: Western blot analysis of CTR and P1 fibroblasts treated with resveratrol. CTR and P1 cells were treated with resveratrol at the concentration of 25 µM for 24 h. Proteins were separated by SDS-PAGE and probed with the following antibodies: ezrin (1 : 2000, Santa Cruz, sc-20773), alpha B crystallin (1 : 1000, Proteintech, 15808-1-AP), peroredoxin-1 (1 : 2000, Santa Cruz, sc-7381), peroredoxin-2 (1 : 2000, Santa Cruz, sc-33572), peroredoxin-6 (1 : 2000, Santa Cruz, sc-393024), and heat-shock protein (HSP70) (1 : 2000, Sigma, H51747). a-Tubulin was used as loading control (1 : 4000, Santa Cruz, sc-23948). This image is representative of three independent experiments.

Fig 4: Correlation between CRYAB expression and immune infiltration in CRC.Correlations between CRYAB expression and different immune cells: (A) neutrophils, (B) macrophages, (C) CD4+T cells, and (D) CD8+T cells. Correlations between CRYAB expression and different immune-related genes: (E) CD2, (F) CD2D, and (G) CD2E.

Fig 5: Differential expression and survival analysis of the CRYAB gene.(A) CRYAB mRNA expression in different cancers; red and blue represent downregulation and upregulation, respectively. (B) Comparative expression of CRYAB mRNA between colorectal tumor tissues and normal tissues; red and blue represent tumor and normal, respectively (statistical significance calculated by difference analysis, *P < 0.05; **P < 0.01; ***P < 0.001). (C) Human CRYAB expression levels in different cancer tissues and corresponding normal tissues. (D–F) The Kaplan–Meier survival curves of CRC patients with high and low CRYAB expression levels. (G) A meta-analysis with two data sets. High expression of CRYAB is significantly associated with poor OS. TE: estimated treatment effect; seTE: standard error of treatment estimate.