Fig 1: Immunofluorescence micrographs of retinal arteriole cross-sections (cryosections) stained with a primary antibody (Abcam, Waltham, MA, USA, dilution 1:200, incubation time: 2 h at RT) targeted against NOX2 (ab80508), NOX4 (ab154244), and NOX5 (ab191010) and a rhodamine Red-X-coupled secondary antibody (Dianova GmbH, Hamburg, Germany, 111-295-003, dilution 1:200, incubation time: 2 h at RT) (red color). Quantification of fluorescent intensities in the vascular wall of retinal arterioles normalized to NG. (A) Immunoreactivity to NOX2 was similar in all groups. (B) In contrast, immunoreactivity to NOX4 was elevated in the HG group. (C) Likewise, immunoreactivity to NOX5 was increased in the HG group. Data are expressed as mean ± SE (n = 8 per group; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Scale bar = 50 µm.