Western blot analysis of HeLa cell lysate (Lane 2) and HepG2 cell lysate (Lane 3) run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane, then probed with Rabbit anti Human GAPDH antibody (AHP1628) at 1/1000 dilution followed by horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (STAR208P) at 1/5000 dilution. Bio-Rad Precision Plus molecular weight markers are run in lane 1. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure.
Western blot analysis of HeLa cell lysate (Lane 2) and HepG2 cell lysate (Lane 3) run under reducing conditions on SDS PAGE using the and transferred to a PVDF membrane, then probed with Rabbit anti Human GAPDH antibody at 1/1000 dilution followed by horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (STAR208P) at 1/5000 dilution. molecular weight markers are run in lane 1. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure.
Supplier Page from Bio-Rad for GOAT ANTI RABBIT IgG (H/L):HRP