Fig 1: Promoter methylation level, genetic alteration and correlation analysis of hnRNPs were evaluated in human colorectal adenocarcinoma. (A, B) In UALCAN database, we found that hnRNPA1, hnRNPA2B1, hnRNPF, and hnRNPK had higher promoter methylation levels in COAD tissues, while hnRNPU had lower promoter methylation levels. In READ tissues, hnRNPC, hnRNPK, hnRNPF, and hnRNPU had lower promoter methylation levels. (C) The hnRNPs mutations and correlations were analyzed by using the cBioPortal database. The data showed that hnRNPs were altered in 119 samples out of 382 colorectal adenocarcinoma patients (31%). Two or more alterations were found in almost a third of the samples (36 samples). (D) The distribution patterns of the alteration frequency of hnRNPs in colorectal adenocarcinoma were presented. Specifically, the predominant form of genetic alteration occurred in COAD and READ was mRNA upregulation, followed by mRNA downregulation, amplification, and deep deletion. (E) The correlations of the eight hnRNPs mRNA levels were analyzed. Pearson’s correlation coefficients revealed that the mRNA levels of hnRNPs with each other were positively correlated.