Anti-MBP Monoclonal Antibody E8032S from New England Biolabs

Product Specs
ItemAnti-MBP Monoclonal Antibody
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE8032S
HostMurine
Conjugate/TagUnconjugated
TargetMBP
Quantity0.05 ml
IsotypeIgG2a
ClonalityMonoclonal
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeAntibody
Storage Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Identification of a novel, tunable interface in the S.pombe HP1 protein, Swi6, that underpins epigenetic inheritancebioRxivDecember 1, 2023Amanda Ames, Melissa Seman, Ajay Larkin, Gulzhan Raiymbek, Ziyuan Chen, Alex Levashkevich, Julie Suzanne Biteen, Kaushik RagunathanSDS-PAGE and transferred to a PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592, Sigma). Western blot data for in vitro binding assays were analyzed

(2) The leucine zipper domain of the transcriptional repressor Opi1 underlies a signal transduction mechanism regulating lipid synthesisJournal of Biological ChemistryDecember 1, 2023J. Pedro Fernández-Murray, Mahtab Tavasoli, Jason Williams, Christopher R. McMasterRRID:AB_10979611) were from Thermo Fisher Scientific. Antibody against MBP was from New England Biolabs (cat# E8032S; RRID:AB_1559730). Antibody against GFP was from Takara (cat# 632380; RRID:AB_10013427). Antibody against

(3) The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticusNature CommunicationsNovember 29, 2023Erica W. Carter, Orlene Guerra Peraza, Nian Wangand western blot (WB) of the GST and MBP fusion proteins using anti-GST (ab92, Abcam, 1:1000 dilution) and anti-MBP (E8032S, NEB, 1:10,000 dilution) monoclonal antibodies before proceeding. The GST-fusion protein and empty-GST

(4) Evolution of a plant growth-regulatory protein interaction specificityNature PlantsOctober 30, 2023Zhe Ji, Eric J. Belfield, Siyu Zhang, Jacques Bouvier, Shan Li, Jason Schnell, Xiangdong Fu, Nicholas P. Harberdmin and detected through immunoblots using anti-His (Santa Cruz, SC-8036, 1:2,000), anti-MBP (NEB, E8032S, 1:10,000) and anti-GST (MBL, PM013-7, 1:5,000) antibodies. Relevant primer sequences are listed in

(5) An IRE1-proteasome system signalling cohort controls cell fate determination in unresolved proteotoxic stress of the plant endoplasmic reticulumNature PlantsAugust 10, 2023Dae Kwan Ko, Joo Yong Kim, Ethan A. Thibault, Federica Brandizzisc-8017), anti-T7 (Sigma-Aldrich; catalogue no. AB3790) and anti-MBP (New England BioLabs; catalogue no. E8032S) sera. The expression and purification of MBP–PIR1ΔN protein were followed using the manufacturer’s

(6) Hem25p is a mitochondrial IPP transporterbioRxivJuly 25, 2023Jonathan Tai, Rachel M. Guerra, Sean W. Rogers, Zixiang Fang, Laura K. Muehlbauer, Evgenia Shishkova, Katherine A. Overmyer, Joshua J. Coon, David J. Pagliarinianti-Coq1 (custom made at Genscript, 1:2000), anti-Vdac1 (Abcam ab110326, 1:4000), and Anti-MBP (NEB E8032S, 1:10000). Secondary antibodies include IRDye 680RD goat anti-rabbit (LI-COR 926–68071, 1:15000), IRDye

(7) Oligomerization and Adjuvant Activity of Peptides Derived from the VirB4-like ATPase of Clostridioides difficileBiomoleculesJune 18, 2023Julya Sorokina, Irina Sokolova, Mariya Majorina, Anastasia Ungur, Vasiliy Troitskiy, Amir Tukhvatulin, Bogdan Melnik, Yury Belyi(cloning with the NH2-terminal glutathione-S-transferase (GST), GE Healthcare). Anti-MBP antibody (#E8032S) was purchased from New England Biolabs, and anti-FLAG antibody (#14793) from Cell Signaling (Leiden,

(8) GCNA is a histone binding protein required for spermatogonial stem cell maintenanceNucleic Acids ResearchJune 9, 2023Jonathan Ribeiro, Gerry P CrossanNacalai USA); anti-beta-Actin (1:2000, catalog no. ab8227, Abcam); anti-MBP (1:10 000, catalog no. E8032S, New England Biolabs); anti-CCNA2 (1:1000, catalog no. ab181591, Abcam); anti-VINCULIN (1:2000, catalog

(9) Maturation of the malarial phosphatidylserine decarboxylase is mediated by high affinity binding to anionic phospholipidsJournal of Biological ChemistryMay 1, 2023Jae-Yeon Choi, Lauren Lopes, Choukri Ben Mamoun, Dennis R. Voelkerbuffer. The proteins bound to the coated lipids were detected with anti-MBP monoclonal antibody (NEB, E8032S, diluted by 30,000-fold in the blocking buffer) and then HRP-IgG–conjugated secondary antibody (Sigma Aldrich

(10) Brassinosteroids modulate autophagy through phosphorylation of RAPTOR1B by the GSK3-like kinase BIN2 in ArabidopsisAutophagyApril 3, 2023Ching-Yi Liao, Yunting Pu, Trevor M. Nolan, Christian Montes, Hongqing Guo, Justin W. Walley, Yanhai Yin, Diane C. Bassham2X SDS sample buffer. Each MBP-tagged RAPTOR1B fragment was detected with anti-MBP antibodies (NEB, E8032S). For the in vitro kinase assay, each MBP-tagged RAPTOR1B fragment, or MBP alone as control, was incubated

(11) Transportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting proteinThe Journal of biological chemistryMarch 1, 2023Pörschke M, Rodríguez-González I, Parfentev I, Urlaub H, Kehlenbach RH.(58), and anti-importin 13 (18) antibodies were described previously. Anti-MBP (New England Biolabs, E8032S), anti-NOSIP (Sigma Aldrich, HPA062132), anti-importin 7 (Thermo Fisher Scientific, PA5-115423), anti-importin

(12) Mechanism of NAIP—NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5Nature Structural & Molecular BiologyFebruary 1, 2023Bhaskar Paidimuddala, Jianhao Cao, Grady Nash, Qing Xie, Hao Wu, Liman Zhangflag peptide for anti-flag resin). Samples were subjected to immunoblotting with anti-MBP (NEB no. E8032S) and anti-Flag (Sigma no. F1804), and all experiments were repeated three times. ATPase activity assay

(13) Phos-tag-based non-radioactive protocols for monitoring Arabidopsis kinase activities in vitroSTAR ProtocolsDecember 1, 2022Junjie Shi, Manru Fang, Ran Wang, Ziqiang Zhu,Affinity Biosciences,Cat# T0007; RRID: AB_2839414 Anti-MBP Monoclonal Antibody (1:10000 dilution),New England Biolabs,Cat# E8032S; RRID: AB_1559730 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, BL21

(14) SYP72 interacts with the mechanosensitive channel MSL8 to protect pollen from hypoosmotic shock during hydrationNature CommunicationsDecember 1, 2022Xuemei Zhou, Yifan Zheng, Ling Wang, Haiming Li, Yingying Guo, Mengdi Li, Meng-xiang Sun, Peng Zhaonitrocellulose membranes (Millipore) for immunoblotting with mouse anti-MBP (NEB; Catalogue number: E8032S; 1:1000 dilution) and mouse anti-Myc (Abclonal; Catalogue number: AE010; 1:1000 dilution). To compare

(15) Abscisic acid regulates stomatal production by imprinting a SnRK2 kinase-mediated phosphocode on the master regulator SPEECHLESSScience advancesOctober 7, 2022Yang X, Gavya S L, Zhou Z, Urano D, Lau OS.antibodies used in the immunoblotting are as follows: monoclonal anti-MBP antibody (New England Biolabs, E8032S, 1:10,000 dilution), monoclonal, horseradish peroxidase (HRP)–conjugated, anti-GST antibody (B-14) (Santa Cruz Biotechnology

(16) The Xanthomonas type-III effector XopS stabilizes CaWRKY40a to regulate defense responses and stomatal immunity in pepper (Capsicum annuum)The Plant CellApril 26, 2022Margot Raffeiner, Suayib Üstün, Tiziana Guerra, Daniela Spinti, Maria Fitzner, Sophia Sonnewald, Susanne Baldermann, Frederik Börnkeperoxidase-conjugated; Santa Cruz Biotechnology Inc.; cat. no. sc-138 HRP) or anti-MBP antibody (1:10,000; NEB, cat. no. E8032S; used with 1:10,000 secondary horseradish peroxidase-conjugated anti-mouse antibody; ThermoFisher; cat.

(17) HOIL‐1 ubiquitin ligase activity targets unbranched glucosaccharides and is required to prevent polyglucosan accumulationThe EMBO JournalApril 19, 2022Ian R Kelsall, Elisha H McCrory, Yingqi Xu, Cheryl L Scudamore, Sambit K Nanda, Paula Mancebo‐Gamella, Nicola T Wood, Axel Knebel, Stephen J Matthews, Philip Cohenantibodies: anti‐HOIP (#SAB2102031) and anti‐HOIL‐1 (#HPA024185) were from Sigma‐Aldrich; anti‐MBP (#E8032S) was from New England Biolabs; HRP‐conjugated anti‐rabbit secondary (#7074) and HRP‐conjugated anti‐mouse

(18) Plant AFC2 kinase desensitizes thermomorphogenesis through modulation of alternative splicingiScienceApril 15, 2022Jingya Lin, Junjie Shi, Zhenhua Zhang, Bojian Zhong, Ziqiang ZhuAntibody,Affinity Biosciences,Cat# T0007; RRID: AB_2839414 Anti-MBP Monoclonal Antibody,New England Biolabs,Cat# E8032S; RRID: AB_1559730 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, DH5α

(19) Fission yeast paxillin contains two Cdc15 binding motifs for robust recruitment to the cytokinetic ringMolecular Biology of the CellApril 1, 2022Chloe E. Snider, Rahul Bhattacharjee, Maya G. Igarashi, Kathleen L. Gouldpolyclonal antibody (VU326; Roberts-Galbraith et al., 2009) or anti-MBP mouse monoclonal antibody (E8032S; New England Biolabs) were used as primary antibodies in immunoblotting. Secondary antibodies were conjugated

(20) Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's diseaseScience advancesApril 1, 2022Pirooznia SK, Wang H, Panicker N, Kumar M, Neifert S, Dar MA, Lau E, Kang BG, Redding-Ochoa J, Troncoso JC, et al.polyubiquitin modifications of PARIS. Anti–maltose binding protein (MBP) (1:1000; New England Biolabs, E8032S) was used for detecting MBP-tagged recombinant Tc PINK1. The Mitochondrial Marker Antibody Sampler Kit

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7/29/2016Anti-MBP Purification Tag Antibody Nico MeyerMBP affinity purification tag can be easily detected using this antibody and therefore used as loading or interaction control in various experimetns such as heterologous protein expresssion and ... Read Review

Anti-MBP Monoclonal Antibody

Anti-MBP Monoclonal Antibody from New England Biolabs

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New England Biolabs

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Anti-MBP Monoclonal Antibody from New England Biolabs

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