Fig 1: Isolation of SARS-CoV-2 RBD-specific human monoclonal antibodies (hmAbs)(A) Representative gating strategy for RBD+ B cell isolation. Initial plots are gated on live CD3-CD4- CD19+ annexin V-CD27+ B cells.(B) hmAbs were tested at 10 and 1 µg/mL in duplicate by ELISA for binding to indicated protein; area under the curve (AUC) is indicated.(C) hmAbs were tested at 10 µg/mL in duplicate for binding to indicated protein by ELISA in the presence or absence of 8 M urea treatment, mean and standard error presented.(D) Binding to SARS-CoV-2 RBD was determined by surface plasmon resonance (SPR), mean and standard deviation presented.
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