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Antibodies
Anti-Osteopontin antibody
Anti-Osteopontin antibody from Abcam
Be the first to write a review!
Citations:
(52)
Supplier Page
Supplier Page from
Abcam for
Anti-Osteopontin antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Osteopontin antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab63856
Antibody Type
Primary
Isotype
IgG
Host
Rabbit
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Clonality
Polyclonal
Quantity
100 UG
Conjugate/Tag
Unconjugated
Concentration
0.3 mg/ml
Gene Name
SPP1
Reactivity
Human, Mouse, Rat
Target
Osteopontin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (52)
(1)
Single‐cell and spatial transcriptomics reveal POSTN + cancer‐associated fibroblasts correlated with immune suppression and tumour progression in non‐small cell lung cancer
Clin Transl Med
December 19, 2023
Chao Chen, Qiang Guo, Yang Liu, Qinghua Hou, Mengying Liao, Yanying Guo, Yupeng Zang, Fei Wang, Huanyu Liu, Xinyu Luan,
et al.
order: Alexa Fluor 647‐conjugated anti‐CD8 antibody (ab305048, Abcam,
1:100
), anti‐osteopontin antibody (
ab63856
,
Abcam
,
1:200
)/TSA 520, anti‐periostin (
hpa012306
,
Merck
)/TSA 620, anti‐CD4 antibody (
ab133616
,
Abcam
,
1:500
)
(2)
A novel adhesive dual-sensitive hydrogel for sustained release of exosomes derived from M2 macrophages promotes repair of bone defects
Materials Today Bio
November 2, 2023
Xiaoying Zhao, Ximiao Chen, Yuxin Deng, Chenyu Wu, Zihang Ruan, Chenchao Li, Yu Chen, Chaoyong Bei, Linyong Zhu, Huachen Yu,
et al.
,
Western blot
,"#BS-
7158
R, Bioss, China" Anti-RUNX2,
Western blot
,"#
ab76956
,
Abcam
, USA" Anti-Osteopontin,
Western blot
,"#
ab63856
,
Abcam
, USA" Anti-β-catenin,
Western blot
/IF,"#
17565-1-AP
,
Proteintech
, China" Anti-β-actin,
Western blot
(3)
Geometric Angles and Gene Expression in Cells for Structural Bone Regeneration
Advanced Science
November 1, 2023
Juan Wang, Qianhao Yang, Qimanguli Saiding, Liang Chen, Mingyue Liu, Zhen Wang, Lei Xiang, Lianfu Deng, Yixuan Chen, Wenguo Cui
cells were fixed, permeabilized, and blocked, they were incubated with a primary antibody against OPN (
ab63856
;
Abcam
), followed by
incubation
with secondary antibodies, phalloidin, and DAPI successively. Finally,
(4)
ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts
Orthopaedic Surgery
September 1, 2023
Guohui Zhou, Xinmin Yan, Zhenfei Chen, Xing Zeng, Fangqian Wu
antibodies against GAPDH (
ab9485
;
1:2500
;
Abcam
), ASPN (
ab31303
;
1:1000
; Abcam), osteopontin (OPN;
ab63856
;
1:1000
;
Abcam
), osteocalcin (OCN;
ab93876
;
1:1000
;
Abcam
), collagen type I alpha 1 chain (
COL1A1
;
ab260043
;
(5)
ZIF-8 induced hydroxyapatite-like crystals enabled superior osteogenic ability of MEW printing PCL scaffolds
J Nanobiotechnology
August 10, 2023
Bingqian Wang, Yuyang Zeng, Shaokai Liu, Muran Zhou, Huimin Fang, Zhenxing Wang, Jiaming Sun
to incubate
membranes
on day 5, whereas those against OCN (
sc-390
,877,
1:500
,
Santa Cruz
) and OPN (
ab63856
,
1:1000
,
Abcam
) were added to incubate
membranes
on day 14. Later, secondary antibodies (
1:10000
) were
(6)
Can locally applied risedronate be an effective agent when combined with xenografts? An animal study
BMC Oral Health
July 24, 2023
Taha Özer, Vusala Guliyeva, Alper Aktaş, Emre Barış, Mert Ocak
sections washed with PBS, the sections were incubated with the primary antibodies anti-osteopontin (
ab63856
Abcam
) and
Anti-Bone Sialoprotein
(
ab52128
Abcam
) at + 4 ℃ overnight. The samples were then washed with
(7)
In situ activation of flexible magnetoelectric membrane enhances bone defect repair
Nature Communications
July 10, 2023
Wenwen Liu, Han Zhao, Chenguang Zhang, Shiqi Xu, Fengyi Zhang, Ling Wei, Fangyu Zhu, Ying Chen, Yumin Chen, Ying Huang,
et al.
skimmed milk), anti-
BMP2
(
ab214821
,
1:1000
, diluted with 5% w/v skimmed milk) and anti-Osteopontin (
ab63856
,
1:1000
, diluted with 5% w/v skimmed milk) were purchased from
Abcam
. The primary antibody anti-β-actin
(8)
3D Biomimetic Calcified Cartilaginous Callus that Induces Type H Vessels Formation and Osteoclastogenesis
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
June 1, 2023
Qiu M, Li C, Cai Z, Li C, Yang K, Tulufu N, Chen B, Cheng L, Zhuang C, Liu Z,
et al
.
EMCN (PA5‐115178,
Invitrogen
,
1:200
),
Alkaline phosphatase
(
NB110‐3638
,
Novus
,
1:200
), Osteopontin (
ab63856
,
Abcam
,
1:200
),
Osterix
(
sc‐393060
,
Santa Cruz Biotechnology
,
1:200
). After primary antibody
incubation
,
(9)
Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D3 Concentration
International Journal of Molecular Sciences
May 12, 2023
Usman Rashid, Sandra K. Becker, Gerhard Sponder, Susanne Trappe, Mansur A. Sandhu, Jörg R. Aschenbach
markers
NT5E
(
CD73
,
ab137595
), ENG (CD105, MA5-11854),
SP7
(osterix,
ab209484
), SPP1 (osteopontin;
ab63856
), and osteocalcin (
ab198228
). All primary polyclonal antibodies were raised in rabbits and procured
(10)
Network pharmacology identifies fisetin as a treatment for osteoporosis that activates the Wnt/β-catenin signaling pathway in BMSCs
Journal of Orthopaedic Surgery and Research
April 22, 2023
Guihong Liang, Jinlong Zhao, Jianke Pan, Yuan Yang, Yaoxing Dou, Weiyi Yang, Lingfeng Zeng, Jun Liu
primary protein antibodies were as follows: anti-
RUNX2
(
1:1000
,
12556
,
CST
), anti-Osteopontin (
1:1000
,
ab63856
,
Abcam
), anti-collagen I (
1:1000
,
ab260043
,
Abcam
), anti-β-actin (
1:2000
, 3700, CST), anti-β-catenin
(11)
Mechanically conditioned cell sheets cultured on thermo-responsive surfaces promote bone regeneration
Biomater Transl
March 28, 2023
Gen Wang, Zhangqin Yuan, Li Yu, Yingkang Yu, Pinghui Zhou, Genglei Chu, Huan Wang, Qianping Guo, Caihong Zhu, Fengxuan Han,
et al.
I,
Mouse
,
1:1000
,
ab6308
,AB_305411,"
Abcam
, Cambridge, UK" Runx2,Rabbit,
1:1000
,
ab76956
,AB_156595,"
Abcam
, Cambridge, UK" OPN,Rabbit,
1:1000
,
ab63856
,AB_1524127,"
Abcam
, Cambridge, UK" β-Actin,
Mouse
,
1:1000
,
ab8226
,AB_306371,"
Abcam
, Cambridge, UK" Goat
(12)
Effects of a locally administered risedronate/autogenous bone graft combination on bone healing in a critical-size rabbit defect model
Journal of Orthopaedic Surgery and Research
February 4, 2023
Taha Özer, Vusala Guliyeva, Alper Aktaş, Emre Barış, Mert Ocak
Scientific) was applied to the tissue sections, and primary antibodies were incubated using anti-osteopontin (
ab63856
Abcam
) and anti-
bone
sialoprotein antibodies (
ab52128
Abcam
) at 4 °C for one night. Washing was subsequently
(13)
PHF8 promotes osteogenic differentiation of BMSCs in old rat with osteoporosis by regulating Wnt/β-catenin pathway
Open Life Sciences
December 2, 2022
Feng Pan, Kai Huang, Hongbin Dai, Chunhe Sha
UK)"
RUNX2
,Rabbit,"
1:1,000
",
ab236639
,
Abcam
ALP
,Rabbit,"
1:1,000
",
ab133602
,
Abcam
Osx
,Rabbit,"
1:1,000
",
ab209484
,
Abcam
OPN,Rabbit,"
1:1,500
",
ab63856
,
Abcam
Wnt5a
,Rabbit,"
1:1,000
",
ab227229
,
Abcam
β-Catenin,Rabbit,"
1:1,000
",
ab32572
,
Abcam
Lamin B1,Rabbit,"
1:2,000
",PB9611,"Boster
(14)
Nano artificial periosteum PLGA/MgO/Quercetin accelerates repair of bone defects through promoting osteogenic − angiogenic coupling effect via Wnt/ β-catenin pathway
Materials Today Bio
December 1, 2022
Xi He, Wenbin Liu, Yanling Liu, Kai Zhang, Yan Sun, Pengfei Lei, Yihe Hu
BSA in 0.1% PBST for 1 h, 1/100 diluted primary antibody collagen I (ab260043Abcam, USA) and OPN (
ab63856
,
Abcam
, USA) were applied and incubated overnight at 4 °C. 1/200 diluted Goat anti-Rabbit IgG (H +
(15)
Palmitic acid inhibits vascular smooth muscle cell switch to synthetic phenotype via upregulation of miR-22 expression
Aging (Albany NY)
October 15, 2022
Yanchao Hu, Yajie Fan, Chunyan Zhang, Congxia Wang
vimentin (
1:2000
; #
ab92547
;
Abcam
), collagen I (
1:1000
; #ab270993; Abcam), osteopontin (OPN;
1:1000
; #
ab63856
;
Abcam
),
LAMC1
(
1:1000
; #
ab233389
;
Abcam
), EVI1 (
1:1000
; #
SAB2100723
;
Sigma
),
AKT3
(
1:2000
; #
ab152157
;
(16)
ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription
Journal of orthopaedic surgery and research
September 19, 2022
Zhu X, Yan F, Liu L, Huang Q.
USA),
ZEB1
(
1:1000
,
GTX33589
,
GeneTex
),
ALP
(
1:2000
,
ab154100
,
Abcam
, Cambridge, MA, USA), OPN (
1:1000
,
ab63856
,
Abcam
),
RUNX2
(
1:1000
,
GTX00792
,
GeneTex
), MMP9 (
1:1000
, ab283575, Abcam),
c-Fos
(
1:2000
.
GTX129846
,
(17)
Extracellular vesicles derived from bone marrow mesenchymal stem cells loaded on magnetic nanoparticles delay the progression of diabetic osteoporosis via delivery of miR-150-5p
Cell Biology and Toxicology
September 16, 2022
Chen Xu, Zhaodong Wang, Yajun Liu, Bangguo Wei, Xiangyu Liu, Keyou Duan, Pinghui Zhou, Zhao Xie, Min Wu, Jianzhong Guan
ab76956
,
Abcam
), BSP (
1:1000
, rabbit, PA5-114,915, Thermo Fisher Scientific), OPN (1 µg/mL, rabbit,
ab63856
,
Abcam
),
OCN
(1–10 µg/mL,
mouse
, MA1-20,786, Thermo Fisher Scientific), and β-actin (
1:5000
,
mouse
,
(18)
The antioxidant enzyme Peroxiredoxin-1 controls stroke-associated microglia against acute ischemic stroke
Redox biology
August 1, 2022
Kim S, Lee W, Jo H, Sonn SK, Jeong SJ, Seo S, Suh J, Jin J, Kweon HY, Kim TK,
et al
.
0.5% Tween 20 and immunolabeled overnight at 4 °C with primary antibodies against osteopontin (
Abcam
,
ab63856
), β-actin (
Santa Cruz
,
sc47778
), or GAPDH (GeneTex, GTX-100118). The appropriate HRP-conjugated secondary
(19)
Inhibition of miR‑98‑5p promotes high glucose‑induced suppression of preosteoblast proliferation and differentiation via the activation of the PI3K/AKT/GSK3β signaling pathway by targeting BMP2
Molecular Medicine Reports
July 29, 2022
Feng Zheng, Fucai Zhang, Furong Wang
ab236639
;
Abcam
), anti-collagen I (
1:1,000
; cat. no.
ab138492
;
Abcam
), anti-OPN (
1:1,000
; cat. no.
ab63856
;
Abcam
), anti-
OCN
(
1:1,000
; cat. no.
ab133612
;
Abcam
), anti-phosphorylated (p)-
PI3K
(
1:1,000
; cat. no.
(20)
Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair
Frontiers in Bioengineering and Biotechnology
March 24, 2022
Senlin Chai, Jianhao Huang, Abdurahman Mahmut, Bin Wang, Yao Yao, Xiaofeng Zhang, Zaikai Zhuang, Chunmei Xie, Zhihong Xu, Qing Jiang
Alizarin Red S and Masson’s Trichrome Stain Kit were purchased from Solarbio. Anti-Osteopontin antibody (
ab63856
) and Anti-Osteocalcin antibody (
ab93876
) were purchased from
Abcam
.
Cell Culture
Rat bone marrow-derived
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Anti-Osteopontin antibody from Abcam
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